This is a review of analytical methods, such as spectrophotometry, derivative spectrophotometry and various chromatographic (gas chromatography-GC, high-performance liquid chromatography-HPLC, thin-layer chromatography-TLC, high-performance thin-layer chromatography-HPTLC, liquid chromatography-tandem mass spectrometry-LC-MS, microchip electrophoresis-MCE, capillary electrophoresis-CE) and elec...

Bennett RD and Heftmann E (1962) Thin-layer chromatography of sterols. Journal of Chromatography 9: 359. Copius-Peereboom JW and Beekes HW (1965) The analysis of mixtures of animal and vegetable fats. V. Separation of sterol acetates by thin-layer chromatography in reversed-phase systems and on silica gel G} silver nitrate layers. Journal of Chromatography 17: 99. Hung GWC and Harris AZ (1989) ...

1064 Identification of Articles of Botanical Origin by High-Performance ThinLayer Chromatography Procedure. High-performance thin-layer chromatography (HPTLC) is widely used for botanical identification. Variability of the chromatographic conditions has the potential to significantly impact the results. This informational general chapter describes, with illustrations, the need to control the re...

A simple, accurate, and precise HPTLC method has been developed and validated for the estimation of efavirenz from bulk drug and capsule formulations. The separation was achieved on TLC plates using an appropriate solvent system. The spots so developed were densometrically scanned at 247 nm. The linearity of the method was found to be within the concentration range of 1-40 μg/ml. The validation...

Frey HP and Zieloff K (1992) Qualitative und quantitative Du( nschicht-Chromatographie. Weinheim: VCH. Fried B and Sherma J (eds) (1996) Practical Thin Layer Chromatography: A Multidisciplinary Approach. Boca Raton, FL: CRC Press. Jork H, Funk W, Fischer W and Wimmer H (1990) Thin Layer Chromatography, vol. 1a. Physical and Chemical Detection Methods. Weinheim: VCH. Jork H, Funk W, Fischer W an...

Medicinal plants containing essential bioactive ingredients are used to cure disease or disorder since time immortal. One of the aims of medicinal plant research is the isolation and identification of markers/ bioactive compounds. Isolation of the markers compounds and bioactive plant constituents has always been a challenging task for the researchers. Separation of these components from the me...