The 5' region of a cell cycle regulated human H1 histone gene appears to contain at least six promoter DNA elements that are shared with some, but not all human core (H2A, H2B, H3 and H4) histone genes. We show that two of these elements represent separate binding sites for two distinct, partially purified factors. The first promoter domain contains A/T rich repeats and is involved in the bindi...

Proof. First we prove the “if” direction. Fix agent 1 and preferences ✓1. Suppose that S1 is not obviously dominant in G = hH, , A,A, P, c, (Ii)i2N , gi. We need to demonstrate that there exists G̃ that is i-indistinguishable from G, such that G,G̃(S1) is not weakly dominant in G̃ . We proceed by construction. Let (S0 1, I1, h sup, S 1 , d sup c , hinf , Sinf 1, d inf c ) be such that I1 2 ↵(S1, S...

Extensive digestion of Chinese hamster metaphase chromosomes with Alu I, Hae III and Hinf I released up to 40 distinct chromosomal proteins. Some of the proteins released by Hae III or Hinf I were enriched in the protein moiety liberated by Alu I but several proteins released by Hae III were not released by Alu I digestion. The amount of chromosomal protein released by deoxyribonuclease I (DNas...

This paper is a preliminary description of three HVR-DNA markers routinely used in Poland: D7S21, D12S11, and D5S110. The distribution of phenotypes and allele frequencies in the population of central Poland is presented. The independence of inheritance within and between loci was confirmed. Our data indicate that among the screening systems the most polymorphic system is D12S11/Hinf I.

The restriction fragment length polymorphism of the 195 bp repeated DNA sequence of Trypanosoma cruzi was analyzed among 23 T. cruzi stocks giving a reliable picture of the whole phylogenetic variability of the species. The profiles observed with the enzymes Hinf I and Hae III were linked together and supported the existence of two groups. Group 1 shows a 195 bp repeated unit (Hinf I) and high ...

The dynamic response characteristics of modern aircraft vary substantially with ight conditions. These changes require scheduling of the ight control system with variables such as dynamic pressure and Mach number. This scheduling can be accomplished easily for simple controllers but is much more diicult for complex controllers which result from the use of most modern control design techniques. ...

Polymorphism. A Hinf I PCR-RFLP was identified in the porcine leptin receptor ( LEPR) gene. Source and Description of Primers. Human cDNA (GenBank accession no. U43168) sequence was used to design primers to amplify porcine genomic DNA. Primer Sequences. Forward primer: 5¢GCATCCCATATCTGAACCC3¢; reverse primer: 5¢-CCACTTAAACCATAGCGAATC3¢.

Conventional alkaline C-banding was found to be an effective additional step in restriction endonuclease banding to improve staining contrast and to reveal hidden changes in the heterochromatic blocks. Examples of these cases are demonstrated on the bovine chromosome set using Hinf I and Taq I endonucleases in the digestion experiments.

BACKGROUND & OBJECTIVE Conventional identification of a clinical isolate of mycobacteria primarily based on culture characteristics and biochemical tests needs several weeks and may remain inconclusive. This study was undertaken to develop a new rapid method to identify the mycobacterial isolates at species level by gene amplification restriction analysis using primers encoding 16S-23S rRNA int...

Electron microscopy was used to study the formation of random complexes between Escherichia coli RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) and a promoterless fragment (Mbo I-C) of bacteriophage T7 DNA, and to determine the location of the polymerase molecules bound at 3 degrees to the promoter-containing (Hinf)1100 fragment of the same DNA. The value of the ...