The techniques of microscopic histochemistry, successfully applied to problems in histopathology, necessarily result in increased objectivity. Combined with procedures for quantification the increase may be such as to remove subjective judgment altogether. Extended to the ultrastructural level the quality of the information derived is greatly enhanced in terms of localization but there is usual...

In this paper, we review the life and work of Mieczyslaw Konopacki and his wife Bronislawa Konopacka. These Polish scientists introduced histochemical methods to embryological studies at the beginning of the XXth century; they were pioneers in this regard in Poland and among the first in the world to implement such methods in this way.

Enzyme histochemistry is frequently used in classical morphological studies for the qualitative analysis of neuronal networks. However, this procedure does not readily provide quantitative results. Two new alternative approaches based on digital image processing techniques were explored and the data quality compared. The preliminary results explored the feasibility of these approaches in the ap...

T H E aim of histoand cytochemical studies is to identify and eventually to quantitate substances on a microscopic level. This necessitates isolating extremely small areas for analysis, preferably in the intact biological tissue. Physical methods have proven to be of value in this respect, in particular the radiant energy micro-absorption techniques (Fitzgerald & Engstrom, 1952). The field of h...