The health and vigour of honeybee colonies are threatened by numerous parasites (such as Varroa destructor and Nosema spp.) and pathogens, including viruses, bacteria, protozoa. Among honeybee pathogens, viruses are one of the major threats to the health and well-being of honeybees and cause serious concern for researchers and beekeepers. To tone down the threats posed by these invasive organis...

Chronic bee paralysis virus (CBPV) infection causes chronic paralysis and loss of workers in honey bee colonies around the world. Although CBPV shows a worldwide distribution, it had not been molecularly detected in Japan. Our investigation of Apis mellifera and Apis cerana japonica colonies with RT-PCR has revealed CBPV infection in A. mellifera but not A. c. japonica colonies in Japan. The pr...

Migratory and local bees in Massachusetts were analyzed for seven viruses. Three were detected: black queen cell virus (BQCV), deformed wing virus (DWV), and sacbrood virus (SBV). DWV was most common, followed closely by BQCV and then by SBV. BQCV and SBV were present at significantly higher rates in the migratory bees assayed, bringing into question the impact that these bees have on the healt...

Viruses are one of the most potential risk factors that negatively affect different life stages honey bees. This study was conducted to determine presence infections caused by deformed wing virus (DWV), black queen cell (BQCV), and acute bee paralysis (ABPV) in bees beekeeping regions Burdur, along with obtaining information about their prevalence this particular region. In our study, adult wer...

Abstract Surveys were conducted in Malatya and Elazığ provinces of Eastern Türkiye between 2018–2019 to detect the presence distribution Deformed wing virus (DWV), Acute bee paralysis (ABPV) Black queen cell (BQCV) infecting honey bees Varroa mites. Thirty mites feeding on 147 collected from twenty-five apiaries fifteen Elazığ. The samples subjected reverse transcriptase-polymerase chain reacti...

Nucleotide sequence analyses were used to identify acute bee paralysis virus (ABPV) and Kashmir bee virus (KBV) isolated from a single honey bee colony. Most of the bees in this colony carried KBV. Some individual bees also carried ABPV, a coexistence not yet seen between these two viruses. Implications of coinfection on viral efficacy are discussed, along with a restriction enzyme assay that c...

Abstract In this study, we conducted a six-month survey to evaluate the current status of three common honey bee viruses (black queen cell virus [BQCV], acute paralysis [ABPV], and sacbrood [SBV]) in Turkey’s Bingöl region revealed their phylogenetic relationships with same types other parts world. We randomly sampled 384 worker bees from total 128 apiaries different locations region. Molecular...

Increasing knowledge of the association of honeybee viruses with other honeybee parasites, primarily the ectoparasitic mite Varroa destructor, and their implication in the mass mortality of honeybee colonies has resulted in increasing awareness and interest in honeybee viruses. In addition the identification, monitoring and prevention of spread of bee viruses is of considerable importance, part...

22 Detection of Chronic bee paralysis virus (CBPV) is reported for the first time in two 23 species of ants (Camponotus vagus and Formica rufa) and in Varroa destructor. A 24 quantitative real-time PCR (qPCR) method was used to detect and quantify CBPV in 25 infected bees, ants and mites. A minus-strand-specific RT-PCR was used to assess viral 26 replication. These results suggest a new way by ...

Abstract The hygienic behavior in honey bees is a complex polygenic trait that serves as natural defense mechanism against bacterial and fungal brood diseases Varroa destructor mites infesting cells. aim of this study was to evaluate the effect dietary amino acids vitamins supplement “BEEWELL AminoPlus” on Apis mellifera colonies combating microsporidial viral infections. experiment performed d...