A pair of degenerate primers, GMPF1 and GMPR1, was designed on the basis of alignment of previously reported Grapevine fanleaf virus (GFLV) movement protein (MP) nucleotide sequences from Iran and other parts of the world. cDNA was synthesized by the use of Oligo d(T)18 from total RNA extraction from each diseased grapevine leaf sample and subjected to polymerase chain reaction (PCR) with the d...

Decent hot-start effects were here reported in Taq DNA polymerase-based polymerase chain reaction (PCR) when water-soluble CdTe quantum dots (QDs) were employed. The hot-start effects were revealed by the higher amplicon yields and distinguished suppression of nonspecific amplification after pre-incubation of PCR mix with quantum dots between 30 ̊C and 56 ̊C. DNA targets were well amplified even ...

This study determined the effects of several variables on the in situ signal after PCR amplification in fixed cells. A signal was evident in all human peripheral blood monocytes fixed in buffered formalin using primers for the human proto-oncogene bcl-2 with in situ PCR only after prolonged fixation and protease digestion. A much lower detection rate was noted after ethanol or acetone fixation ...

The polymerase chain reaction (PCR) is an indispensable DNA amplification technique that has been exploited in numerous areas, including molecular diagnostics. One major drawback of PCR is the competing amplification of undesired off-target products, which primarily occurs at ambient temperatures prior to PCR cycling (1). Hot Start PCR techniques aim to block the extension of primers in nonspec...

The JHU HLTCOE participated in the Cold Start task in this year’s Text Analysis Conference Knowledge Base Population evaluation. This is our third year of participation in the task, and we continued our research with the KELVIN system. We submitted experimental variants that explore use of forward-chaining inference, slightly more aggressive entity clustering, refined multiple within-document c...

This study investigates the morphology and nanostructure of soot particles during cold-start hot-start engine operation a diesel using oxygenated fuels. The samples were analysed transmission electron microscopy. oxygen content in fuel was varied between 0 12%. results showed that primary have significantly smaller size when compared to operation. addition fuels also resulted sized particles. S...