Availability of rapid and reliable methods for detection of quality in plant raw materials and botanicals is urgently needed. The recent HPTLC instrumentation allows to obtain fingerprints useful to ascertain identity and composition. The results of direct application of HPTLC devices in selected cases, using the fingerprint approach, are here reported, considered and compared with other method...

The aim of the work is to develop a simple, rapid, selective and cost effective HPTLC method for the determination of galangin in Alpinia galanga & Alpinia officinarum. The HPTLC densitometric technique was therefore, selected for the quantitative and qualitative determination of galangin in Alpinia galanga and Alpinia officinarum respectively. There are different analytical methods were used t...

Liquid-liquid extraction-thin layer chromatography (LLE-TLC) has been a common and routine combined method for detection of drugs in biological materials. Solid-phase extraction (SPE) is gradually replacing the traditional LLE method. High performance thin layer chromatography (HPTLC) has several advantages over TLC. The present work studied the higher efficiency of a new SPE-HPTLC method over ...

High performance thin layer chromatography (HPTLC) combined with on-spot detection and characterization via easy ambient sonic-spray ionization mass spectrometry (EASI-MS) is applied to the analysis of biodiesel (B100) and biodiesel-petrodiesel blends (BX). HPTLC provides chromatographic resolution of major components whereas EASI-MS allows on-spot characterization performed directly on the HPT...

High-performance thin-layer chromatography (HPTLC)-bioassays are promising new methods for detecting bioactive chemicals in food packaging. Here, we test whether direct-acting genotoxic detectable contact materials (FCM) using HPTLC-bioassays. First, an interactive worksheet lays out steps to calculate needed detection limits (bio)analytical from regulatory limits, including thresholds of toxic...

Abstract High-performance thin-layer chromatography (HPTLC), as the modern form of TLC (thin-layer chromatography), is suitable for detecting pharmaceutically active compounds over a wide polarity range using gradient multiple development (GMD) technique. Diode-array detection (DAD) in conjunction with HPTLC can simultaneously acquire ultraviolet?visible (UV?VIS) and fluorescence spectra direct...

Six natural polyphenolic compounds, brevifolin carboxylic acid, brevifolin, ellagic acid, methyl gallate, gallic acid and protocatechuic acid have been isolated from the methanol extract of the whole plant of Erodium cicutarium (L.) L.'Hérit. (Geraniaceae). Structures were determined by conventional methods of analysis and confirmed by MS and NMR spectral analysis. The distribution of these com...

In this study, we have chosen HPTLC as a method of analysis to develop a standard procedure based on fingerprinting characteristics for the evaluation of homoeopathic formulations. A simple and accurate HPTLC method has been developed for the quantification of Arjunolic acid and fingerprinting of the in-house mother tincture considered here to be a standard with that of different marketed sampl...

Objective: To establish the fingerprint profile of Wedelia chinensis using thin layer chromatography (TLC) and high performance thin layer chromatography (HPTLC) technique. Methods: TLC and HPTLC studies were carried out in two different solvent systems, which showed different Rf value. Results: TLC profiling of the extract confirm about the presence of various phytochemicals. HPTLC finger prin...

In this study, we have chosen HPTLC as a method of analysis to develop a standard procedure based on fingerprinting characteristics for the evaluation of homoeopathic formulations. A simple and accurate HPTLC method has been developed for the quantification of BacosideA and fingerprinting of the in-house mother tincture considered here to be a standard with that of different marketed samples av...