Significance Human cytomegalovirus (HCMV) reactivation is a major cause of posttransplant morbidity/mortality. One approach toward reducing this purging the transplant donor and/or recipient latently infected cells prior to stem-cell or organ harvest/engraftment. Our findings show involvement host bromodomain (BRD) proteins in regulation HCMV latency and reactivation. Bromodomain extra-terminal...

Background and Aims: Primary infections of rubella and human cytomegalovirus (HCMV) can lead to severe complications in pregnancy. The screening of Rubella and HCMV in pregnant women is not routinely carried out in Iran. This cross sectional study aimed to investigate the seroprevalence of HCMV and rubella infections in neonates with and without congenital defects. Materials and Methods: This s...

The human cytomegalovirus (HCMV) is a global opportunistic β-herpes virus causing severe diseases in immune-compromised patients, such as malignant tumor especially those undergoing chemotherapeutic treatment. This study aimed to determine the prevalence of HCMV-DNA treatment naive cancer and after chemotherapy, compare between conventional nested PCR ELISA techniques for detection HCMV, detect...

BACKGROUND Breastfeeding imparts beneficial immune protection and nutrition to infants for healthy growth, but it is also a route for human immunodeficiency virus (HIV) and human cytomegalovirus (HCMV) infection. In previous studies, we showed that HCMV adversely affects infant development in Africa, particularly with maternal HIV exposure. In this study, we analyzed infants risks for acquisiti...

Despite a lot of research, the etiology and progression of breast cancer remain incompletely understood. Recently, human cytomegalovirus (HCMV) was reported as a risk factor for breast cancer. The aim of this study was to know whether breast cancer could be caused by cytomegalovirus or not? In this experiment seventeen samples of RAZI/A mice with spontaneous breast cancer were being gathered fr...

Human cytomegalovirus (HCMV) DNA quantitation in whole blood (WB) by real-time or quantitative polymerase chain reaction (qPCR) is a highly sensitive and reproducible diagnostic procedure for monitoring HCMV DNAemia (DNAemia the detection of samples plasma, blood, isolated peripheral leukocytes buffy-coat specimens) patients. We provided comparative analysis extraction performance two different...

BACKGROUND The immunomodulatory drug leflunomide (LEF) is frequently used for treating human cytomegalovirus (HCMV), but its antiviral mechanism is still unclear. In this study,we therefore investigated the effects of the active LEF metabolite A771726 on the HCMV lifecycle in human embryonic lung fibroblasts. We clarified the mechanism of LEF antiviral infection, and provide a new way to treat ...

List of original publications 7 Abbreviations 8 Summary 9 Review of the literature 10 1. Human cytomegalovirus 10 1.1. Virus structure and genome organization 10 1.2. Virus replication 10 1.3. Epidemiology and transmission 11 1.4. Pathogenesis 12 2. Human cytomegalovirus infections in organ transplant patients 14 2.1. Clinical manifestations 14 2.2. Prevention and treatment of HCMV disease 15 2...

Previous studies indicated that elevated serum levels of Interleukin -18 (IL-18) and human cytomegalovirus (HCMV) antibodies have a fundamental role in the pathogenesis Type I diabetic mellitus T1DM. Therefore, aim current study was to investigate possible overlap between Interleukin-18 with The (45) T1DM patients healthy controls were used detect IL-18 an ELISA Kit HCMV cobas c411 apparatus fo...

Accurate and rapid diagnosis of human cytomegalovirus (HCMV) disease in immunocompromised patients has remained as a challenge. Quantitative competitive PCR (QC-PCR) methods for detection of HCMV in these individuals have improved the positive and negative predictive values of PCR for diagnosis of HCMV disease. In this study we used QC-PCR assay, using a co-amplified DNA standard, to quantitate...