Aspergillus oryzae koji plays a crucial role in fermented food products due to the hydrolytic activities of secreted enzymes. In the present study, we performed a comparative secretome analysis of the industrial strain of Aspergillus oryzae 3.042 and its spontaneous mutantZJGS-LZ-21. One hundred and fifty two (152) differential protein spots were excised (p<0.05), and 25 proteins were identifie...

Paracoccidioides brasiliensis is a thermally dimorphic fungus that causes paracoccidioidomycosis. The yeast form of this pathogen is found in the animal host whereas the mycelial form is recovered from living and non-living organic material. The sole carbon source available in these habitats is represented by polysaccharides from the plant cell wall. Hydrolytic enzymes are necessary to convert ...

A histochemical study of hydrolytic enzymes such as acid phosphatase and beta-glucuronidase was made on cultured brain tumor cells. Well-differentiated astrocytic cells, oligodendroglioma cells, and medulloblastoma cells showed low activities. Migrating cells and macrophage-like cells showed intense activities. Meningioma cells and schwannoma cells showed relatively intense activities at the ea...

Mycophenolate mofetil (MMF) is the ester prodrug of the immunosuppressant agent mycophenolic acid (MPA) and is rapidly activated by esterases after oral administration. However, the role of isoenzymes in MMF hydrolysis remains unclear. Although human plasma, erythrocytes, and whole blood contain MMF hydrolytic activities, the mean half-lives of MMF in vitro were 15.1, 1.58, and 3.20 h, respecti...

Thermochemical modification of lysozyme enables it to retain its oligomers, showing a wide spectrum of increased antibacterial activities. It also results in significant loss of hydrolytic activity of the enzyme. The objective of this study is to analyse the protective effect of resorcinol on the hydrolytic activity of modified lysozyme using a high temperature method and the oligomerization de...

We studied the possibility that the antagonistic saprobe fungi Coriolopsis rigida, Trametes versicolor, Fusarium lateritium, Penicillium chrysogenum and Verticillium dahliae-2379 may control V. dahliae disease through the inhibition of the activity of hydrolytic enzymes produced by the pathogen. These saprobe fungi were able to decrease the growth in vitro of V. dahliae. The exudates produced b...

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