The possibility of hydroxylamine electrochemical determination over a CoO(OH)-modified electrode, accompanied by complex-formation, has been studied from the theoretical point of view. The correspondent mathematical model has been developed and analyzed by means of linear stability theory and bifurcation analysis. It was shown that CoO(OH) may be applied as an efficient electrode modifier i...

In the present paper, the electrochemical properties of magnetic iron oxide nanoparticles (Fe2O3 and Fe3O4) as highly sensitive sensors for the simultaneous determination of hydrazine and hydroxylamine was described. The electrochemical behavior of hydrazine and hydroxylamine was investigated using cyclic voltammetry, chronoamperometry and differentia...

Hydroxylamine has been determined by its oxidation to nitrite with a known excess of bromine. Bromine in acidic medium bleaches the dye methyl red. A known excess of bromine when treated with hydroxylamine is reduced to bromide and the unreacted bromine is determined using methyl red. The method obeys Beer’s law in the range 0-5 μg of hydroxylamine in an overall aqueous volume of 25 mL. The rel...

(+)- And (-)-amphetamine and methamphetamine were N-oxygenated by the cDNA expressed adult human flavin-containing monooxygenase form 3 (FMO3), their corresponding hydroxylamines. Two major polymorphic forms of human FMO3 were studied, and the results suggested preferential N-oxygenation by only one of the two enzymes. Chemically synthesized (+/-)-amphetamine hydroxylamine was also a substrate ...

[adenine-U-14C]ADP-ribose-agmatine and [adenineU-’4C]ADP-ribose-histone were synthesized by an NAD:arginine ADP-ribosyltransferase from [’*C]NAD and agmatine and histone, respectively. The pseudofirst order rate constants for breakdown of the two components either in 0.4 N NaOH or in 0.4 M neutral hydroxylamine were identical. Hydroxylamine treatment of [‘4C]ADP-ribose-agmatine or [32P]ADP-ribo...

The intermediate role of hydroxylamine in nitrate assimilation has been suggested by observations that different organisms form ammonia from hydroxylamine in vivo, and that hydroxylamine occurs in nutrient media and cells. In further support are recent investigations describing enzymatic preparations which catalyze the reduction of hydroxylamine to ammonia. Taniguchi et al. (J. Biochem. (Japan)...

The one pot reaction of glyoxal and hydroxylamine hydrochloride in aqueous sodium hydroxide was found to be a safe and inexpensive method for the preparation of diaminoglyoxime. By increasing stoichiometric ratio of the hydroxylamine hydrochloride and decreasing the solvent volume, the product yield increased considerably (~ 70%).

The kinetic characteristics of the diaphorase activities associated with the NADH-dependent nitrite reductase (EC 1.6.6.4) from Escherichia coli have been determined. The values of the apparent maximum velocity are similar for the reduction of Fe(CN)6(3)-and mammalian cytochrome c by NADH. These reactions may therefore have the same rate-limiting step. NAD+ activates NADH-dependent reduction of...