In soybean seeds the level of hydroxyproline is regulated in a developmental and tissue-specific manner. The seed coat contains approximately 77% of the total hydroxyproline in the seed at all stages of development. We determined the ratio of hydroxyproline to dry weight in a number of tissues within the seed; however, only the seed coat shows an increase in this ratio during development. Withi...

A study has been made of the hydroxyproline-induced inhibition of elongation of Avena coleoptile tissues. The isomers of 4-hydroxyproline differ in their effectiveness; only the L isomers are growth inhibitors with the cis form (allohydroxyproline) being more effective than the trans form (hydroxyproline). Hydroxyproline differs from other amino acid antagonists and protein synthesis inhibitors...

Urinary glycosaminoglycan and hydroxyproline excretion was studied in 11 patients with clear evidence of Paget's disease of bone. Urinary hydroxyproline, cetyl pyridinium chloride (CPC)-precipitable uronic acid and CPC-precipitable hexosamine were expressed as ratios to urinary creatinine. Urine samples were concentrated x 1000 by vacuum dialysis and the glycosaminoglycans examined by electroph...

The discovery of bound hydroxyproline in higher plants (1, 2) and the demonstration that this hydroxyproline is in peptide linkage (2, 3) and localized in the primary cell wall (3-8) shows that study of the metabolism and role of hydroxyproline can no longer be restricted to the field of collagen studies. Previous work (3-6) indicates that the wall-bound hydroxyproline is derived from free prol...

To determine the quantitative relationship of urinary hydroxyproline peptide excretion to collagen breakdown, known quantities of radioactive hydroxyproline peptides were administered to unlabeled animals and excertion of radioactivity in respiratory carbon dioxide, urine, and feces was measured. The major routes of excretion of collagen peptide metabolites were respiratory carbon dioxide (75%)...

Incubation of cartilage from chick embryos with l*C3,5-cis-hydroxy-DL-proline demonstrated that cis-hydroxyproline is incorporated into the collagen and other protein synthesized by the tissue. Gel filtration of extracts from the tissue demonstrated that the proteins synthesized in the presence of cis-hydroxyproline were about the same size as proteins synthesized under control conditions. Incu...

1. After the administration of labelled proline to guinea pigs deprived of ascorbic acid for 15 days, the dorsal skin was examined 5 days later in an attempt to detect the presence of hydroxyproline-deficient collagen (protocollagen). The extent of incorporation of proline into skin collagens indicated a severe impairment of collagen synthesis. 2. A comparison of proline and hydroxyproline spec...

A study has been made of the distribution and metabolism of protein-bound hydroxyproline in an elongating tissue, the excised Avena coleoptile. The hydroxyproline-containing proteins of this tissue have been separated into 3 fractions on the basis of their solubilities. The cytoplasmic, trichloroacetic acid-insoluble proteins (S-fraction) contain the bulk of the proline of the cells but only 20...