objective(s): the present study was aimed to investigate the influence of thoracic epidural blockade on hypoxia-induced pulmonary hypertension in rats. materials and methods: forty eight wistar rats were randomly divided into 4 equal groups, named normoxia hypoxia hypoxia/ ropivacaine and hypoxia/saline. animals were placed in a hypoxia chamber and instrumented with epidural catheters at the th...

OBJECTIVE Pulmonary hypertension remains a major clinical problem despite current therapies. In this study, we examine for the first time a novel pharmacological target, smooth muscle myosin, and determine if the smooth muscle myosin inhibitor, CK-2019165 (CK-165) ameliorates pulmonary hypertension. MATERIALS AND METHODS Six domestic female pigs were surgically instrumented to measure pulmona...

BACKGROUND Evidence suggestive of endoplasmic reticulum (ER) stress in the pulmonary arteries of patients with pulmonary arterial hypertension has been described for decades but has never been therapeutically targeted. ER stress is a feature of many conditions associated with pulmonary arterial hypertension like hypoxia, inflammation, or loss-of-function mutations. ER stress signaling in the pu...

Hypoxia exposure often cause the increases of pulmonary arterial pressure (PAP). Studies reported that mast cells (MCs) participate in vascular remodeling and promote formation chronic hypertension. Current studies mainly focus on change MCs under hypoxia, but few regulatory role mechanism acute hypoxia. T?herefore, present study investigated dynamic lung tissues hypoxia activation increasement...

Hypoxia induced pulmonary hypertension (HPH) represents a complex pathology that involves active vascular remodeling, loss of vascular tone, enhanced pulmonary inflammation, and increased deposition of extracellular matrix proteins. Megakaryocytic leukemia 1 (MKL1) is a transcriptional regulator known to influence cellular response to stress signals in the vasculature. We report here that in re...

Objective: Pulmonary arterial hypertension is characterized by abnormal proliferation of pulmonary artery smooth muscle cells and vascular remodeling, which leads to right ventricular (RV) failure. Bsg (Basigin) a transmembrane glycoprotein that promotes myofibroblast differentiation, cell proliferation, matrix metalloproteinase activation. CyPA (cyclophilin A) binds its receptor inflammatory r...

Patients treated with hemodialysis (HD) repeatedly undergo intradialytic low arterial oxygen saturation and central venous saturation, reflecting an imbalance between upper body systemic supply demand, which are associated increased mortality. Abnormalities along the entire cascade, impaired diffusive convective transport, contribute to reduced tissue supply. HD treatment impairs pulmonary gas ...

Pulmonary arterial hypertension (PAH) is an intractable disease of the small pulmonary artery that involves multiple inflammatory factors. We hypothesized that a redox-sensitive transcription factor, nuclear factor B (NFB), which regulates important inflammatory cytokines, plays a pivotal role in PAH. We investigated the activity of NFB in explanted lungs from patients with PAH and in a rat mod...

Pulmonary hypertension (PH) is characterized by profound vascular remodeling and altered Ca 2+ homeostasis in pulmonary arterial smooth muscle cells (PASMCs). Magnesium ion (Mg ), a natural antagonist cofactor for numerous enzymes, crucial regulating diverse cellular functions, but its roles PH remains unclear. Here, we examined the of Mg transporters development. Chronic hypoxia monocrotaline ...

BACKGROUND Mutations in the KCNK3 gene have been identified in some patients suffering from heritable pulmonary arterial hypertension (PAH). KCNK3 encodes an outward rectifier K(+) channel, and each identified mutation leads to a loss of function. However, the pathophysiological role of potassium channel subfamily K member 3 (KCNK3) in PAH is unclear. We hypothesized that loss of function of KC...