A protocol was developed for callus induction and regeneration in tomato (Lycopersicon esculentum) var. Moneymaker. Hypocotyl and leaf disc explants of tomato were used as a starting material for callus induction. Explants were cultured on MS medium having different concentrations of hormones. Maximum callogenesis from hypocotyls was obtained on MS medium supplemented with IAA (2 mg/l), NAA (2 ...

Ruta graveolens L. is a source of pharmacologically active molecules such as furocoumarins, furoquinolines and acridone alkaloids. A genetic transformation method using Agrobacterium tumefaciens was developed for this plant species. The conditions for an efficient regeneration of R. graveolens led us to retain hypocotyls among other explants tested. Subsequently, transformation was obtained by ...

In vitro morphogenic response of mature seed embryo-derived callus cultures Chonemorpha fragrans was studied using solid and liquid Murashige Skoog medium amended with cytokinins or their combinations naphthalene acetic acid at 0.5 mg L-1. The plant growth regulators (PGRs) combination concentrations tested could not stimulate organogenesis after three subcultivations the on same PGRs-amended w...

The improvement of previously described protocols for the regeneration shoots from ‘Canino’ mature seed hypocotyl slices has been accomplished. effects different factors such as part used, vacuum-infiltration, 2,4-Dichlorophenoxyacetic acid pulse, vacuum-infiltration and sonication on transient transformation were analyzed. When three obtained hypocotyls evaluated separately medium, highest per...

Background: Ferulago angulata is an important medicinal plant and endemic to Iran which is an endangered Plant. Objective: The aim of this study was to achieve an efficient method for propagation of this plant and evaluation of essential oil content and composition in habitat. Metthods: Essential oils were extracted by hydrodistillation using Clevenger-type apparatus and its composition was ...

Callus-forming capacity is enhanced with hypocotyl maturity in Arabidopsis. However, the genetic regulation of age-related gain in capacity for callus formation is unclear. We used a gene expression microarray assay to characterize the underlying mechanisms during callus formation in young and mature hypocotyl explants of Arabidopsis. As expected, genes involved in photosynthesis and cell wall ...

Tomato (Lycopersicon esculentum Mill.) as the most economically important vegetable crop worldwide has been investigated intensively for development of new and improved varieties. Most these technologies require efficient protocols in vitro regeneration propagation plant material. In present study, an reproducible system five Romanian tomato genotypes (cvs. ‘Capriciu’, ‘Darsirius’, ‘Kristin’, ‘...

The present work describes the effects of winter and spring genotypes of rapeseed (PF7045/91, Okapi and Opera), explants (cotyledons and hypocotyls), light, different kinds and concentrations of auxins [Naphthylacetic acid (NAA) and 2, 4dichlorophenoxyacetic acid (2, 4-D)], presence of cytokinin [benzyladenine (BA)] and different concentrations of micro and macro elements (MS and 1/2MS) on call...