Atomic force microscopy reveals that Pseudomonas aeruginosa LecA (PA-IL) and a tetra-galactosylated 1,3-alternate calix[4]arene-based glycocluster self-assemble according to an aggregative chelate binding mode to create monodimensional filaments. Lectin oligomers are identified along the filaments and defects in chelate binding generate branches and bifurcations. A molecular model with alternat...

Die beiden tetravalenten Lektine LecA und LecB des ESKAPE-Erregers Pseudomonas aeruginosa sind notwendig für dessen bakterielle Adhäsion, Biofilmbildung Invasion in Wirtszellen. Sie gelten daher als Ansatzpunkte zur medikamentösen antiinfektiösen Therapie. Vor kurzem berichteten wir über hochwirksame divalente Galaktoside spezifische LecA-Inhibitoren. Diese litten jedoch unter einer sehr gering...

Readily accessible, low-valency glycoclusters based on a triazine core bearing D-galactose and L-fucose epitopes are able to inhibit biofilm formation by Pseudomonas aeruginosa. These multivalent ligands are simple to synthesize, are highly soluble, and can be either homofunctional or heterofunctional. The galactose-decorated cluster shows good affinity for Pseudomonas aeruginosa lectin lecA. T...

The bacterial adhesion lectin LecA is an attractive target for interference with the infectivity of its producer P. aeruginosa. Divalent ligands with two terminal galactoside moieties connected by an alternating glucose-triazole spacer were previously shown to be very potent inhibitors. In this study, we chose to prepare a series of derivatives with various new substituents in the spacer in hop...

Entamoeba invadens is not the secondarily reduced and simplified eukaryote unrelated to a cenancestor as thought by some protist researchers. On the contrary, E. invadens is a single-celled eukaryote with a complex life cycle comprised of stem cells and stem cell lines. It contains hypoxic and oxygenic life cycle stages revealing to us details about the living conditions of LECA at the time of ...

The presence of a nucleus and other membrane-bounded intracellular compartments is the defining feature of eukaryotic cells. Endosymbiosis accounts for the origins of mitochondria and plastids, but the evolutionary ancestry of the remaining cellular compartments is incompletely documented. Resolving the evolutionary history of organelle-identity encoding proteins within the endomembrane system ...

The eukaryotic endomembrane system (ES) is served by hundreds of dedicated proteins. Experimental characterization of the ES-associated molecular machinery in several model eukaryotes complemented by a recent progress in phylogenomics and comparative genomics have revealed a conserved complex core of the machinery that appears to have been established before the last eukaryotic common ancestor ...

A pea genomic library was screened with a pea seed lectin cDNA clone, a selected clone sub-cloned and the DNA sequence determined. We have designated this gene LecA; like other lectin genes LecA contains no introns. The coding sequence of this gene is identical to that of the cDNA used to select it, pLGA.10 (1) and that of the published cDNA sequence (2). Although these cDNAa differ in their si...

As a nucleolar complex for small-subunit (SSU) ribosomal RNA processing, SSU processome has been extensively studied mainly in Saccharomyces cerevisiae but not in diverse organisms, leaving open the question of whether it is a ubiquitous mechanism across eukaryotes and how it evolved in the course of the evolution of eukaryotes. Genome-wide survey and identification of SSU processome components...

Pseudomonas aeruginosa (PA) is a major public health care issue due to its ability to develop antibiotic resistance mainly through adhesion and biofilm formation. Therefore, targeting the bacterial molecular arsenal involved in its adhesion and the formation of its biofilm appears as a promising tool against this pathogen. The galactose-binding LecA (or PA-IL) has been described as one of the P...