intradermal injection of autologous serum and plasma elicit a cutaneous reactivity in almost 45-60% of patients with chronic idiopathic urticaria (ciu).   this reactivity is associated with the presence of auto antibodies against ige or ige receptors. this study was carried out to compare the cutaneous reactivity of autologous serum and plasma skin tests in a series of patients with ciu for dia...

background: nasal polyps, a common clinical problem, are characterized by eosinophilic and mast cell inflammation.  the role of allergy and ige in pathogenesis of nasal polyps is still unclear.  ige receptors are important components of the immunological pathway in allergic and inflammatory diseases.   objective: to determine if the low affinity ige receptor (cd23) is presented on nasal polyp t...

Release of pro-inflammatory mediators by mast cells is a key feature of allergic disease. The 'dogma' is that IgE molecules merely sensitise mast cells by binding FcεRI prior to cross-linking by multivalent allergen, receptor aggregation and mast cell activation. However, certain monoclonal IgE antibodies have been shown to elicit mast cell activation in an antigen-independent cytokinergic mann...

Soluble IgE receptors are potential in vivo modulators of IgE-mediated immune responses and are thus important for our basic understanding of allergic responses. We here characterize a novel soluble version of the IgE-binding alpha-chain of Fc-epsilon-RI (sFcεRI), the high affinity receptor for IgE. sFcεRI immunoprecipitates as a protein of ∼40 kDa and contains an intact IgE-binding site. In hu...

Controlled cross-linking of IgE-receptor complexes on the surface of rat basophilic leukemia cells and mast cells has allowed a comparison of the lateral mobility and cell triggering activity of monomers, dimers, and higher oligomers of receptors. Addition of a monoclonal anti-IgE(Fc) antibody to IgE-sensitized cells in stoichiometric amounts relative to IgE produces IgE-receptor dimers with hi...

BACKGROUND Elevated serum immunoglobulin (Ig) E is a diagnostic marker of immediate-type allergic reactions. We hypothesize that serum IgE does not necessarily reflect total body IgE because in vivo IgE can be bound to cell surface receptors such as FcepsilonRI and FcepsilonRII (CD23). The aim of this study was to analyze the relationships between levels of serum IgE, cell-bound IgE, and IgE-re...