Immobilized whole cells of Rhodotorula rubra MIM147 were used in a packed bed flow reactor for the enantioselective reduction ?-ketonitriles and obtainment key building block synthesis antidepressant drug duloxetine.

Enzymatic production of dihydroxyacetone (DHA) was studied by immobilization of the whole cells of acetic acid bacteria capable of oxidizing glycerol to DHA. Acetobacter xylinum A-9 cells immobilized in a polyacrylamide gel were selected as the most favorable enzyme preparation. The enzymatic properties of immobilized cells converting glycerol to DHA were investigated and compared with those of...

entrapment of permeabilized whole cells within a matrix is a common method for immobilization. chitosan possesses distinct chemical and biological properties, which make it a suitable matrix for entrapment and immobilization of penicillin g acylase (pga). in the first step, escherichia coli (atcc 11105) cells were permeabilized using n-cetyl-n,n,n-trimethyl ammonium bromide (ctab) (0.1% w/v, 45...

presently there are a number of fine and sensitive methods for determining chemical or biochemical substance among which bio electrodes represent the most recent development. bioelectrodes consist of biologically active materials (e.g enzyme, antibody, whole cell or cell fragments) held in close proximity to a suitable electrochemical transducer that sense specific electroactive enzyme substrat...

We introduce a new smooth, non-toxic, biocompatible method for cross-linking of gum tragacanth (GT), a polysaccharide of natural origin, in order to serve as a new supporting matrix for immobilization systems.  The modified gum is used as a matrix for the catalysis of the conversion of benzyl penicillin to 6-aminopenicillanic acid (6-APA) by means of Escherichia coli ATCC11105 with penicill...

BACKGROUND AND OBJECTIVES The attempts were made to describe the development of a whole cell immobilization of P. pastoris by entrapping the cells in polyacrylamide gel beads. The alcohol oxidase activity of the whole cell Pichia pastoris was evaluated in comparison with yeast biomass production. MATERIALS AND METHODS Methylotrophic yeast P. pastoris was obtained from Collection of Standard M...