Broad-spectrum analysis for pathogens in patients with respiratory tract infections is becoming more relevant as the number of potential infectious agents is still increasing. Here we describe the new multiparameter RespiFinder assay, which is based on the multiplex ligation-dependent probe amplification (MLPA) technology. This assay detects 15 respiratory viruses in one reaction. The MLPA reac...

The diagnosis of leprosy continues to be based on clinical symptoms and early diagnosis and treatment are critical to preventing disability and transmission. Sensitive and specific laboratory tests are not available for diagnosing leprosy. Despite the limited applicability of anti-phenolic glycolipid-I (PGL-I) serology for diagnosis, it has been suggested as an additional tool to classify lepro...

the present study was conducted to detect prevalence and risk factors for retrovirus infection of infected cats in a cat population in iran, by evaluation of 238 client-owned cats of different ages that were tested for the presence of fiv antibodies. the cats were selected from those referring to veterinary hospital of ahvaz university, southwestern iran from december 2007 to june 2010. classif...

feline infectious peritonitis virus (fipv) is ubiquitous in domestic cats, especially in young cats and multi-cat environments. in the present study, a total of 248 companion cats of different ages were examined for serum antibody detection of fipv by immunochromatography assay. the cats were selected from those referring to veterinary hospital of ahvaz university, southwestern iran from decemb...

BACKGROUND The occurrence of Giardia duodenalis in cats is of potential significance from both clinical and public health perspectives. The object of this study was antigenic detection of G. duodenalis in household cats of Ahvaz district, South-West of Iran. METHODS The prevalence of G. duodenalis was determined in fecal samples by two techniques: centrifugation-flotation and a commercial Gia...

Objective(s) Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of nosocomial and community infections. Biofilm formation, mediated by a polysaccharide intercellular adhesin (PIA) and encoded by the ica operon, is considered to be an important virulence factor in both S. epidermidis and S. aureus. However, the clinical impact of the ica locus and PIA production is less w...

objective(s) methicillin-resistant staphylococcus aureus (mrsa) is an important cause of nosocomial and community infections. biofilm formation, mediated by a polysaccharide intercellular adhesin (pia) and encoded by the ica operon, is considered to be an important virulence factor in both s. epidermidis and s. aureus. however, the clinical impact of the ica locus and pia production is less wel...

background: the occurrence of giardia duodenalis in cats is of potential significance from both clinical and public health perspectives. the object of this study was antigenic detection of g. duo­denalis in house­hold cats of ahvaz district, south-west of iran. methods: the prevalence of g. duodenalis was determined in fecal samples by two techniques: centrifuga­tion-flotation and a commercial ...

BACKGROUND Human serum albumin (HSA) is the most abundant plasma protein and plays key a role in metabolism. The variation in albumin concentration provides valuable information related to metabolic diseases and diagnostic application. METHODS We constructed two assay systems to quantify the albumin concentration. The immunoassay used a fluorescence (FL) dye to detect albumin in samples and e...

We developed an immunochromatography-based assay for detecting antibodies against recombinant α-galactosidase A proteins in serum. The evaluation of 29 serum samples from Fabry patients, who had received enzyme replacement therapy with agalsidase alpha and/or agalsidase beta, was performed by means of this assay method, and the results clearly revealed that the patients exhibited the same level...