A filovirus, serologically related to Ebola virus, was detected by "post-embedment" immunoelectron microscopical examination of MA-104 cells. These had been infected by inoculation with serum samples obtained during the 1989 epizootic in cynomolgus monkeys (Macaca fascicularis), imported from the Philippines and maintained at Reston, Virginia, USA, a primate holding facility. The immunoelectron...

Immunolocalization of antigens in cells requires simultaneous preservation of structure and antigenicity. The difficulties of immunolocalization methods are centered around the methods used to preserve structure, which as a rule tend to degrade the ability of antigens (usually proteins) to be recognized by their cognate antibodies. The value of immunolocalization for cell biology was early reco...