TnphoA was used to mutagenize the chromosome in an effort to identify membrane-bound and exported components of the long-chain fatty acid transport system of Escherichia coli. This strategy identified three classes of fusions that were unable to grow or grew at reduced rates on minimal agar plates containing the long-chain fatty acid oleate (C18:1), (i) fadL-phoA, (ii) tolC-phoA, and (iii) tsp-...

BACKGROUND In recent years, there have been intensive efforts to develop synthetic microbial platforms for the production, biosensing and bio-remediation of fossil fuel constituents such as alkanes. Building predictable engineered systems for these applications will require the ability to tightly control and modulate the rate of import of alkanes into the host cell. The native components respon...

Monomers of microbial polyhydroxyalkanoates, mainly 3-hydroxyhexanoic acid (3HHx) and 3-hydroxyoctanoic acid (3HO), were produced by overexpressing polyhydroxyalkanoates depolymerase gene phaZ, together with putative long-chain fatty acid transport protein fadL of Pseudomonas putida KT2442 and acyl-CoA synthetase (fadD) of Escherichia coli MG1655 in P. putida KT2442. FadL(Pp), which is responsi...

Escherichia coli FadR plays two regulatory roles in fatty acid metabolism. FadR represses the fatty acid degradation (fad) system and activates the unsaturated fatty acid synthetic pathway. Cross-talk between E. coli FadR and the ArcA-ArcB oxygen-responsive two-component system was observed that resulted in diverse regulation of certain fad regulon β-oxidation genes. We have extended such analy...

Hermeneutics is an interpretation method that develops in the study of texts. Its usage horizon Islamic text (Qur’an and Hadith) not exception. One figure who discusses different areas law Khaled M. Abou El-Fadl. has analyzed various texts their relation to people's lives. This attempts explore thoughts El-Fadl, especially regarding hermeneutics analyzing its with religious moderation Indonesia...

Fatty acids (FAs) have numerous functions in all living organisms, ranging from structural roles and energy production to the biosynthesis of secondary metabolites. Because of the high energy content of exogenous FAs, their acquisition is central of metabolism, and several biological systems are known, although their precise roles are not yet entirely clear. We investigated the roles of FadD (C...

Ligand-gated channels, in which a substrate transport pathway is formed as a result of the binding of a small-molecule chemical messenger, constitute a diverse class of membrane proteins with important functions in prokaryotic and eukaryotic organisms. Despite their widespread nature, no ligand-gated channels have yet been found within the outer membrane (OM) of Gram-negative bacteria. Here we ...

Hijab menjadi praktek yang mempengaruhi dinamika sosial politik kompleks. sering bentuk penegasan identitas muslimahnya seorang perempuan. Penelitian ini difokuskan hukum hijab dalam hermeneutika negosiasi Khaled Aboe el Fadl. Mekanisme relasi triadik antara teks, pengarang dan pembaca dasar bahasan negosiasi. Artikel menggunakan metode deskriptif kajian pada bukunya berjudul Atas Nama Tuhan: D...

BACKGROUND Despite evidence that altered membrane porins may impair microbial drug uptake thereby potentially compounding efflux pump-mediated multidrug resistance, few studies have evaluated gene transcription to identify multidrug-resistance-associated porins and other potential drug targets. METHODS Genes that encode six membrane porins (fadL, lamB, ompC, ompF, ompW and yiaT) and two membr...