background:   objective: the objective of this study was to evaluate the effect of rabbit antiserum to es cells on mouse preimplantation embryo development and chimera production.   materials and methods: mouse 4-cell embryos were matured in vitro at 37.5 o c, in humidified 5% co 2 atmosphere for 12-36 h. the embryos were cultured in ksom medium with or without antiserum for 12-36 h. the ratios...

Production of high quality embryos in vitro needs an efficient in vitro fertilization (IVF). Seminal origin is one of the important factors that affects the success of in vitro embryo production. So our goal was to determine the effect of using fresh and frozen semen in fertilization on developmental competence and cryo-survival of buffalo embryos. Buffalo oocytes wer...

Objective A unique feature of embryo metabolism is production of reactive oxygen species (ROS). It is well established that during in vitro culture, ROS levels increase over normal ranges observed for embryos developed in vivo. This study evaluates and compares the stepwise pattern of ROS production during in vitro development of reconstructed goat embryos produced by zona-free method of somati...

This study involves in vitro androgenesis of Zea mays L. via anther culture. In the first experiment, three embryo induction media were tested in ETH-M82 genotype of maize. The results showed that the embryo structures were established 2 to 3 weeks after culturing the anthers. The effect of induction medium was signifi­cant for embryo frequencies. The obtained results confirm that the induction...

Background: Hyaluronan (HA) is a structural component of extracellular matrix synthesised by HA synthases HAS1-3, which produce HA of different molecular sizes with distinct biological functions associated with reproductive processes. Hyaluronidase (HYAL) cleaves the HA into biologically active small fragments which are known to regulate cell proliferation through CD44 receptor signaling. HA is...

background: in this study, we aimed to investigate the ability of transvaginal doppler ultrasonography in predicting in vitro fertilization (ivf) outcome in women undergoing this procedure. materials and methods: in this prospective observational study, 65 infertile women underwent ivf procedure in isfahan, during 2013-2014, were studied. the pulsatility index (pi), resistive index (ri) and pea...

taxus chinensis var. mairei is a rare and endangered medicinal plant species distributed in china. in order to promote fast propagation and preserve the natural resources, conditions for in vitro germination and seedling development of embryos of t. chinensis var. mairei from anhui or zhejiang were investigated. results showed that in vitro germination rate of excised embryos cultured under 14 ...

background: vitamin d in complex with its receptor by regulating gene expression, endometrium immune response and stimulation of endometrium decidualization can be involved in implantation. so, it seems that the amount of vitamin d in follicular fluids (ff) may have an association with art success. objective: first, we intended to investigate the possible association between levels of follicula...

background: the latest studies reported that local endometrial injury is a useful method to improve the success of ivf-icsi outcome. objective: to assess whether local endometrial injury occurred by pipelle in the spontaneous cycle could improve implantation rate, cleavage rate, and pregnancy outcome in the subsequent ivf-icsi cycle in patients who had recurrent ivf failure. materials and metho...

background: recurrent implantation failure is one of the most issues in ivf cycles.some researchers found that beneficial effects of endometrial scratching in womenwith recurrent implantation failure, while some authors demonstrated contrary resultsobjective: the present study aimed to investigate the effect of intrauterine. salineinfusion as a form of endometrial injury, during fresh in vitro ...