Agar substrates for in vitro culture are well adapted to plant micropropagation, but not to plant rooting and acclimatization. Conversely, paper-pulp-based substrates appear as potentially well adapted for in vitro culture and functional root production. To reinforce this hypothesis, this study compares in vitro development of nemesia on several substrates. Strong differences between nemesia ro...

a reliable method is described for rapid proliferation of axillary shoots from nodal segment of mature field-grown aromatic and medicinal plant vitex negundo through in vitro culture. of different concentrations of n6-benzyladenine (ba) evaluated as supplements to murahige and skoog (ms) medium, ba at 1mg/l was effective in enhancing bud break and shoot regeneration. response of explants was al...

Pitaya belongs to the family Cactaceae and genus Hylocereus. It is essential todevelop tissue culture protocols according appropriate variety spread pitayacommercial production ensure healthy sapling production. This study aimed todetermine best plant growth regulators (PGRs) in micropropagation process,and their effects on different pitaya cultivars were evaluated. Shoots of pitayacultivars cu...

A successful protocol was developed for mass propagation of Lawsonia inermis Linn., an important medicinal plant. Multiple shoots were induced in apical and axillary meristems derived from mature explants of L. inermis on Murashige and Skoog (1962) medium supplemented with 0.25 mg/l 6-benzylaminopurine (BA), 0.25 mg/l Kinetin (Kn), 0.5 mg/l ascorbic acid and 3% (w/v) sucrose. The rate of multip...

Hyperhydricity of shoots initiated in vitro, poor shoot extension, inability cultures to maintain good growth over an extended time, and unsuccessful ex vitro rooting have limited the development a commercial scale micropropagation system for hemp ( Cannabis sativa ). We present culture initiation method that prevents hyperhydricity using vented-lid vessels with 0.2-µm pores medium containing a...

A protocol for micropropagation of Boswellia ovalifoliolata Bal & Henry (Burseraceae) was developed using cotyledonary nodal explant on Murashige and Skoog modified medium (MS). A comparative study of micropropagation with 6-benzyladenine, kinetin and thidiazuron along with 1-naphthalene acetic acid (0.054 microM) was conducted. The highest shoot multiplication (7.1 +/- 0.2 shoots per node) was...

The present study was conducted at the Biotechnology Laboratory, Biotechnology Division, Bangladesh Agricultural Research Institute (BARI), Gazipur during the period from September 2004 to June 2005 to investigate the effect of different concentrations of BAP and NAA on virus free plant regeneration, shoot multiplication and different concentrations of IBA and IAA on in vitro root formation of ...

Introduction Koa (Acacia koa) is currently the most important commercialized hardwood in Hawai'r's forests. A sustainable supply of koa will depend on reforestation with the best adapted seed sources and perhaps with vegetatively propagated cultivars having unique traits. At present, koa forests are in a serious state of decline, and improvement appears warranted if sustainable supply is to be ...