More than 50% out of 129 Agave species are endemic to Mexico. Among them, peacockii is among the list threatened that require special protection. In this work, we aimed at developing new supplementary strategies achieve micropropagation and perform cryopreservation in vitro grown shoot-tips A. peacockii. For multiplication, addition two cytokinins, 6-benzylaminopurine (26.6 μM) kinetin (27.84 M...

An efficient protocol for in vitro propagation of Thamnocalamus spathiflorus (Trin.) Munro, an evergreen temperate bamboo, through multiple shoot formation from zygotic embryos excised from germinating seeds, as well as from nodal explants taken from a 2-year-old plant has been developed. Multiple shoot formation was standardized in both the cases on Murashige and Skoog (MS) medium supplemented...

background: spermatogonial stem cells (sscs), a subset of undifferentiated type a spermatogonia, are the foundation of complex process of spermatogenesis and could be propagated in vitro culture conditions for long time for germ cell transplantation and fertility preservation. objective: the aim of this study was in vitro propagation of human spermatogonial stem cells (sscs) and improvement of ...

In this study, prostatic acid phosphatase (PAP), which is overexpressed in human prostate cancer cells, was cloned to be fused to the IgM constant fragment (Fc) for enhancing immunogenicity and expressed in transgenic tobacco plants. Then, the transgenic plants were propagated by in vitro tissue subculture. Gene insertion and expression of the recombinant PAP-IgM Fc fusion protein were confirme...

Ascophyllum nodosum is the most important commercial seaweed. Seaweeds have been applied as soil conditioners in improving plant growth and development in agricultural crops. In current study and for the first time, extracts of brown seaweed A. nodosum improved the growth of Robinia pseudoacasia L., an ornamental tree. This research investigated the effect of cultivation beds (sand, perlite, co...

The antimicrobial activity of 18 different extracts from in vivo and in vitro grown L. album L. plants was evaluated against clinical bacteria and yeasts using the well diffusion method. All the used extracts demonstrated antibacterial activity, whereas only the water extracts from leaves (in vivo) possessed antifungal activity against Candida albicans NBIMCC 72 and Candida glabrata NBIMCC 8673...