The ontogeny of the lens crystallin antigens throughout development of three marine cephalopod embryos, Loligo vulgaris, Sepia officinalis and Octopus vulgaris has been investigated using the indirect immuno-cytochemical staining against respective homologous anti-total lens protein antiserum. The cellular mechanism of lens development appears to be the same in all species investigated and so d...

The monitoring of salivary cortisol as a key biomarker of an individual's stress response has been increasingly focused on. This paper describes the development of a novel cortisol immuno-assay method based on an indirect competitive method using a commercially available surface plasmon resonance instrument. The surface of an Au chip was modified with PEG6-COOH aromatic dialkanethiol self-assem...

A newly developed Enzym Like Immuno Sorbant Assay (ELISA) based on the recombinant nucleocapsid protein (N) of Schmallenberg virus (SBV) was evaluated and validated for the detection of SBV-specific IgG antibodies in ruminant sera by three European Reference Laboratories. Validation data sets derived from sheep, goat and bovine sera collected in France and Germany (n = 1515) in 2011 and 2012 we...

The detection of immunoglobulin M (IgM) antibodies to cytomegalovirus-induced late antigens by an indirect immunofluorescence assay was improved by the use of monoclonal antibodies to human IgM. Nonspecific background fluorescence was absent, facilitating the reading of the slides and the detection of a specific fluorescence reaction in sera with low levels of specific IgM. Moreover, the indire...

Pasteurella multocida (P. multocida) is an important pathogen of various domestic animals. The outer membrane proteins (OMPs) play a major role in pathogenesis and immunogenicity of P. multocida. The aim of the study was to develop indirect enzyme linked immuno sorbant assay (ELISA) based on OMPs to ascertain the antibody titers in animals post-infection or to gauge the potency of vaccine. The ...

Climate change, world population growth, and poverty have led to an increase in the incidence of leptospirosis. Leptospirosis is caused by pathogenic spirochaete bacteria that belong to the genus Leptospira. The bacteria are maintained in the renal tubules of the reservoir hosts (typically a rodent), then shed into the environment via the urine. Water is key for environmental survival and trans...

pasteurella multocida (p. multocida) is an important pathogen of various domestic animals. the outer membrane proteins (omps) play a major role in pathogenesis and immunogenicity of p. multocida. the aim of the study was to develop indirect enzyme linked immuno sorbant assay (elisa) based on omps to ascertain the antibody titers in animals post-infection or to gauge the potency of vaccine. the ...

Objective: Thyroid diseases can be diagnosed and monitored by serum thyroid stimulating hormone (TSH) measurement along with T3 T4 (both free total). However, TSH is used to distinguish between euthyroid hyperthyroid patients. Hence, analysis sensitivity of the assay plays a very significant role. Aims objectives – The aim study was perform precision study, calculate randomerror analytical rang...

This study aimed to diagnose Lumpy skin desease by isolation and identification of LSD from suspected naturally infected cattle its effect on immuno-biochemical parameters, inflammatory oxidative stress markers. The was conducted 25 cattle, 1-3 years age two cities in the Sharkia governorate. Ten clinically healthy represented control group fifteen diseased cases group. clinical signs showed an...