Chemical methods for the determination of reduced ascorbic acid (Zascorbic acid) are based upon its reducing properties. The indophenol method of Bessey (1, 2) and the perinaphthindantrione hydrate method of Moubasher (3) are examples of these methods. Modifications to increase the specificity of the indophenol method have been presented by Robinson and Stotz (4), Lowry, Lopez, and Bessey (5), ...

The ascorbic acid content of selected fruits was collected from a local market Mandya and Maddur in these were determined by volumetric method using 2,6-dichlorophenol indophenol. results obtained confirms the presence fruits. highest yield found Guava lowest different tested. These suggest that wide variety can be consumed to meet daily requirements vitamin C cost-effective manner.

Of the many methods that have been proposed for the direct chemical determination of vitamin C in plant. and animal tissues, those based upon the reduction of 2 ,Bdichlorophenol indophenol have been adopted far more widely than others. The original indophenol procedure proposed by Tillmans and associates (1) a short time before the vitamin was isolated and identified (2) was soon modified in va...

An isolate of Pseudomonas aeruginosa lacking characteristic indophenol oxidase was recovered from a catheterized urine specimen.

Cytidine is an industrially useful precursor for the production of antiviral compounds and a variety of industrial compounds. Interest in the microbial production of cytidine has grown recently and high-throughput screening of cytidine over-producers is an important approach in large-scale industrial production using microorganisms. An enzymatic assay for cytidine was developed combining cytidi...

The Nadi reaction, discovered by Ehrlich (1885), has been used for many years as a test for indophenol oxidase. The reaction consists in the formation of indophenol blue from dimethyl-p-phenylenediamine and cr-naphthol and is given by most animal and plant cells. It is now generally held that indophenol oxidase is identical with cytochrome c oxidase, and the Nadi reaction is often employed for ...

A treatment procedure using lysozyme and ethylenediaminetetracetic acid gave intact but permeable cells (permeaplasts) of Anacystis nidulans. Rates of electron transport from water to carbon dioxide, ferricyanide, 2,6-dichlorophenol indophenol, benzoquinone, and methyl viologen, and from reduced indophenol to methyl viologen were measured as a function of treatment time. Rates of oxygen evoluti...

An automated method for routine determination of nanomolar ammonium in seawater has been developed using segmented flow analysis coupled with a 2-m-long liquid waveguide capillary cell. Conventional photometric detector and autosampler were modified for this method. The optimal concentrations of the reagents and parameters for the development of indophenol blue are discussed. The method has low...