Sequences of the genome that are capable of silencing gene expression are thought to play a key role in gene regulation. However, very few silencer elements capable of functioning in mammalian cells have been described, and only a fraction of these have been tested for the ability to function in an autonomous fashion. We report here the characterization and functional validation of a constituti...

The osteocalcin (OC) silencer is a unique example of exonic sequences contributing to negative transcriptional control of mammalian gene expression. In this paper we demonstrate, using a reporter transfection assay, that multiple elements reside within the OC +24/+151 domain. Thirty-fold repression is mediated by the +49/+104 fragment, experimentally relocated 3' of the poly(A) signal. Deletion...

The potential for acoustic metamaterials to provide a breakthrough in sound attenuation has been unlocked recent times due advancements additive manufacturing techniques. These materials allow the targeting of specific frequencies attenuation. To date have not experimentally tested an automotive silencer performance enhancement. This study investigates effect within representative silencer. res...

Synthetic regulatory proteins such as tetracycline (tet)-controlled transcription factors are potentially useful for repression as well as ectopic activation of endogenous genes and also for probing their regulatory mechanisms, which would offer a versatile genetic tool advantageous over conventional gene targeting methods. In this study, we provide evidence supporting this concept using Cd4 as...

This study is concern about the behavior of a diesel generator sets in terms sound pressure level and drop. Numerical had been carried out by using simulation from ANSYS software to obtain drop across duct silencer sets. Fluent Harmonic Responses was used perform on silencer. The design 3-dimensional model consists splitter rockwool as its porous medium. Observation focused along process detail...

Negative regulation of cytokine gene transcription is an important mechanism in maintaining homeostasis of immune function. In this study, we characterized a silencer element in the human interleukin-3 gene promoter that is responsible for the cell-specific expression of interleukin-3. This silencer activity was proposed to be mediated by an unidentified nuclear inhibitory protein (NIP). In thi...

Significant potential for acoustic metamaterials to provide a breakthrough in sound attenuation has been unlocked recent times due advancements additive manufacturing techniques. These materials allow the targeting of specific frequencies attenuation. To date, have not demonstrated commercial automotive silencer performance enhancement. A significant obstacle practical use is need low cost and ...

Silencing of retrovirus vectors poses a significant obstacle to genetic manipulation of stem cells and their use in gene therapy. We describe a mammalian silencer blocking assay using insulator elements positioned between retrovirus silencer elements and an LCRbeta-globin reporter transgene. In transgenic mice, we show that retrovirus silencers are blocked by the cHS4 insulator. Silencer blocki...