INTRODUCTION: Low-intensity pulsed ultrasound (LIPUS) stimulation has been observed to enhance bone formation, cartilage regeneration, and softtissue (tendon and muscle) healing. In particular, increased biosynthetic activity and chondrogenic differentiation of human mesenchymal stem cells were observed under LIPUS . However, optimal conditions (such as frequency, intensity and treatment durati...

OBJECTIVE We examined the effects of low-intensity pulsed ultrasound (LIPUS) on cell differentiation, bone mineralized nodule formation and core-binding factor A1 (Cbfa1) expression in a normal human osteoblast (NHOst) cell line and bone formation in an osteoporosis animal model. METHODS NHOst cells were cultured in vitro in medium with or without LIPUS stimulation. The ultrasound stimulation...

Introduction: Most fractures in elderly are fragility fractures occurring in osteoporotic bones. Delayed in treatment may cause morbidity and mortality with many complications and social burdens. Our previous clinical study had shown that the use of low intensity pulsed ultrasound (LIPUS) could provide a beneficial effect on complex fracture. This promising efficacy was further consolidated thr...

Lipid-coated microbubbles are used to enhance ultrasound imaging and drug delivery. Here we apply these microbubbles along with low intensity pulsed ultrasound (LIPUS) for the first time to enhance proliferation and chondrogenic differentiation of human mesenchymal stem cells (hMSCs) in a 3D printed poly-(ethylene glycol)-diacrylate (PEG-DA) hydrogel scaffold. The hMSC proliferation increased u...

Low intensity pulsed ultrasound (LIPUS) has been widely used in clinic for the treatment of repairing pseudarthrosis, bone fractures and of healing in various soft tissues. Some reports indicated that LIPUS accelerated peripheral nerve regeneration including Schwann cells (SCs) and injured nerves. But little is known about its appropriate intensities on autograft nerves. This study was to inves...

Over the past few years, tissue engineering applied to the dental field has achieved relevant results. Tissue engineering can be described by actions taken to improve biological functions. Several methods have been described to enhance cellular performance and low intensity pulsed ultrasound (LIPUS) has shown to play an important role in cell metabolism. The present article provides an overview...

The osteochondral defects represent a major health problem. It is therefore important to improve the already established treatment methods and to develop new, more effective therapies. A new promising therapeutic approach offers the mechanobiological osteoinductive effect of low-intensity pulsed ultrasound (LIPUS), already established in the regenerative repair of bone fractures. Ultrasound bas...

Previously, we successfully applied a new method composed of drilling, bone marrow transplantation (BMT), external fixation and low-intensity pulsed ultrasound (LIPUS) for the clinical treatment of Kienböck's disease. The purpose of this study was to investigate whether bone regeneration can be induced by LIPUS and/or multiple drilling and/or BMT within a severely necrotic small-bone rabbit mod...

The objective of this study was to investigate whether low intensity pulsed ultrasound (LIPUS) has anabolic effects on human periodontal ligament (PDL) cells. The PDL cells were plated in 48-well plates and cultured at 37°C in an atmosphere of 5% CO2 in air, in a humidified incubator until confluent. The cells were divided into three groups including control, 5 min and 10 min ultrasound applica...

Although daily low-intensity pulsed ultrasound (LIPUS) can accelerate osteogenic differentiation of the rat clonal cell line ROS 17/2.8, the molecular mechanism that underlies this phenomenon is unclear. The purpose of this study was to determine which molecules exposed to daily LIPUS treatment stimulate osteogenic differentiation. The cells were cultured in the presence and absence (control) o...