Acidophiles maintain near-neutral intracellular pH using proton pumps. We have suggested the protective role of osmolytes and membrane lipids in adaptation to an acidic environment. Previously we observed, for first time, high levels trehalose acidophilic basidiomycete Sistotrema brinkmannii. Here, studied composition both two more fungi. Trehalose polyols were among main during growth under op...

The dopaminergic system has been suggested to serve as a functional target for ketamine, especially with its addictive and antidepressant effects. In this work, intracellular vesicle impact electrochemical cytometry single cell amperometry were applied model line show that the intravesicular contents number of exocytotic events increased fraction release decreased after treatment by subanesthet...

Ether lipids are ubiquitous constituents of cellular membranes with no discrete cell biological function assigned yet. Using fluorescent polyene-ether lipids we analyzed their intracellular distribution in living cells by microscopy. Mitochondria and the endoplasmic reticulum accumulated high amounts of ether-phosphatidylcholine and ether-phosphatidylethanolamine. Both lipids were specifically ...

Pathogenic mycobacteria are able to survive and proliferate in phagosomes within host macrophages (Mphi). This capability has been attributed in part to their cell wall, which consists of various unique lipids. Some of these are important in the host-pathogen interaction, such as resistance against microbicidal effector mechanisms and modulation of host cell functions, and/or are presented as A...

The oxidative modification of LDL seems a key event in atherogenesis and may participate in inflammatory tissue injury. Our previous studies suggested that the process of LDL oxidation by activated human monocytes/macrophages required O2- and activity of intracellular lipoxygenase. Herein, we studied the mechanisms involved in this oxidative modification of LDL. In this study, we used the human...

Abstract Background Leaves constitute a large input of lipids to soil, yet comparatively little is known about the fate leaf in soil. Our aim was explore initial stages degradation lipids, both loss intact lipid and subsequent mineralisation. We focussed on intracellular – triacylglycerols implicated storage, membrane such as phospholipids galactolipids, pigments because they collectively more ...

Roop Mallik studies how molecular motors and lipids interact to drive intracellular transport.

Membrane lipid homeostasis is maintained by de novo synthesis, intracellular transport, remodeling, and degradation of lipid molecules. Glycerophospholipids, the most abundant structural component of eukaryotic membranes, are subject to acyl chain remodeling, which is defined as the post-synthetic process in which one or both acyl chains are exchanged. Here, we review studies addressing acyl ch...