Dear Sir: Recently Lee et al. (1978) and Akaike et al. (1978) have published in this Journa l two very interest ing papers , repor t ing elegant exper iments with the internally per fused nerve cell bodies. We are especially happy with the success of these authors: the idea o f how simple it is to substitute the cytoplasm of the cell body by artificial saline has once more appeared to be frui t...

Dear Sir: Recently Lee et al. (1978) and Akaike et al. (1978) have published in this Journa l two very interest ing papers , repor t ing elegant exper iments with the internally per fused nerve cell bodies. We are especially happy with the success of these authors: the idea o f how simple it is to substitute the cytoplasm of the cell body by artificial saline has once more appeared to be frui t...

Slow Inward Current, Thallium Intoxication, Voltage Clamp, Available Intracellular Energy To conclude our investigation of thallium effects on cardiac tissues, we studied the slow inward current of sheep cardiac Purkinje fibres exposed to 10“7 to 105 m Tl+ for extended periods of up to 80 min. Our previous results had suggested a possible involvement of the slow inward current during thallium i...

An early inward tail current evoked by membrane depolarization (from -80 to -40 mV) sufficient to activate sodium but not calcium current was studied in single voltage-clamped ventricular myocytes isolated from guinea pig hearts. Like forward-mode Na-Ca exchange, this early inward tail current required [Na+]o and [Ca2+]i and is thought to follow earlier reverse-mode Na-Ca exchange that triggers...

The effects of the nitric oxide donor, S-nitrosoacetylpenicillamine (SNAP), were tested on cultured dissociated guinea pig celiac ganglion neurons using whole cell patch-clamp recordings. S-nitrosoacetylpenicillamine induced a concentration- and voltage-dependent inwardly directed shift in holding current (inward current shift) in 89% of neurons. The inward current shift was prevented by pre-tr...

We have used the 2-electrode voltage-clamp technique to analyze the effects of the neuropeptide, egg-laying hormone (ELH), and the biogenic amine 5-HT on ionic currents in the buccal motoneuron B16 of Aplysia. When B16 is voltage-clamped near resting membrane potential, bath-applied ELH induces a prolonged inward shift in holding current. The ELH-induced inward current is not due to a decrease ...

Voltage clamp experiments were carried out with short Purkinje fibers exposed to acetylstrophanthidin. A consistent change in membrane current was an appearance of a transient inward current on repolarization to the resting potential from the preceding depolarization, when preparations were treated with sufficient concentration of acetylstrophanthidin to cause the transient depolarization. This...

We have used the patch clamp technique to characterize whole-cell currents in spheroplasts isolated from a trk1Delta trk2Delta strain of Saccharomyces cerevisiae which lacks high- and moderate-affinity K+ uptake capacity. In solutions in which extracellular divalent cation concentrations were 0.1 mM, cells exhibited a large inward current. This current was not the result of increasing leak betw...