An isopropyl beta-D-thiogalactopyranoside (IPTG)-inducible episomal expression system has been established for the human breast carcinoma cell line MCF7. This two-component system includes: (i) a primate cell-specific episomal vector, pEpiLac, that contains an IPTG-inducible promoter and (ii) a cell line derived from MCF7, MCF7/LAP5, which expresses the IPTG-dependent transactivator LAP267. Tre...

We have constructed a lac repressor/operator-based system to tightly regulate expression of bacterial genes during intracellular infection by Listeria monocytogenes. An L. monocytogenes strain was constructed in which expression of listeriolysin O was placed under the inducible control of an isopropyl-beta-D-thiogalactopyranoside (IPTG)-dependent promoter. Listeriolysin O (LLO) is a pore-formin...

Amphiphilic inducer molecules such as N-acyl-L-homoserine lactones (AHLs) or isopropyl-β-D-thio-galactopyranoside (IPTG) can be utilized for the implementation of an artificial communication system between groups of E. coli bacteria encapsulated within water-in-oil microemulsion droplets. Using spatially extended arrays of microdroplets, we study the diffusion of both AHL and IPTG from inducer-...

A study of the optimization of CuZn superoxide dismutase gene expression in E. coli L21/pET28-SOD was undertaken. The SOD gene was directionally cloned into expression vector pET28.The recombinant plasmid was transformed into E. coli BL21 and induced by IPTG to express SOD protein. The four effective factors, including the initial concentration of BL21/pET28-SOD, concentration of IPTG, incubati...

The effects of lactose, galactose, glucose, and glucose combined with galactose on the induction of β–galactosidase by IPTG in Escherichia coli strain VC10, growing in a glycerol media, were compared against a control of glycerol plus IPTG. After inducing β– galactosidase production with the IPTG, the cultures were altered with the addition of one of the above carbohydrates or combinations. Thi...

Low purification efficiency and incomplete characterization of male goat (buck) spermadhesins (Bdhs) prompted us to develop an effective system to produce recombinant Bdhs (rBdhs). Bdh-2 cDNA was inserted into a prokaryotic expression plasmid, pTrcHis TOPO. The pTrcHis-Bdh-2 system was constructed to produce a His(6) fusion protein in Escherichia coli Top10 cells. Recombinant clones were select...

LLC-PK1, an epithelial cell line derived from the kidney proximal tubule, was used to study the ability of the G protein α-subunit, Gαq, to regulate cell differentiation. A constitutively active mutant protein, αqQ209L, was expressed using the LacSwitch-inducible mammalian expression system. Induction of αqQ209L expression with isopropyl-β-d-thiogalactopyranoside (IPTG) enhanced phospholipase C...

BACKGROUND: Use of lactose-rich concentrates from dairy processes for the induction of recombinant gene's expression has not received much attention although they are interesting low cost substrates for production of recombinant enzymes. Applicability of dairy waste for induction of recombinant genes in Escherichia coli was studied. Clones expressing Lactobacillus phage muramidase and Lactobaci...

A previous research and studies have established the significance of describing Staphylokinase as a thrombolytic treatment, particularly in patients with cardiovascular disease, stroke, other life-threatening disorders. plays significant part coagulation process through formation plasmin-Staphylokinase complex on surface clot which activates plasminogen is generated by some strains Staphylococc...

It was shown earlier that Tn551 inserted into the C-terminal region of murE of parental methicillin-resistant Staphylococcus aureus strain COL causes a drastic reduction in methicillin resistance, accompanied by accumulation of UDP-MurNAc dipeptide in the cell wall precursor pool and incorporation of these abnormal muropeptides into the peptidoglycan of the mutant. Methicillin resistance was re...