histomorphologic and histomorphometric studies on the pancreas of mehraban male sheep were done in four age groups: fetus, neonate, young adult and old age in this respect, we used special stainings, light microscope and ocular micrometer. the results showed that the smooth muscles were absent in capsule. the tubular portion of exocrine pancreas was more prominent. neuroinsular complex and beta...

Objective(s): The use of a co-culture of islets with mesenchymal stromal cells (MSCs) is a promising therapy in islet transplantation to revert hyperglycaemia, but the resulting insulin-producing cells (IPCs) express low levels of pancreas endocrine developmental genes. This study aims to investigate the morphochronology of a co-culture of islets with MSCs from injured adult pancreata, and char...

Background: Pancreatic cancer‐associated diabetes mellitus (PC‐DM) is present in most patients with pancreatic cancer, but its pathogenesis remains poorly understood. Therefore, we aimed to characterize tumor infiltration Langerhans islets cancer and determine clinical relevance. Methods: islet invasion was systematically analyzed 68 ductal adenocarcinoma (PDAC) using histopathological examinat...

Ischemia-driven islet isolation procedure is one of the limiting causes pancreatic transplantation. Ischemia-reperfusion process associated with endothelium dysfunction and release pro-senescent microvesicles. We investigated whether endothelial microvesicles prompt senescence in vitro. Pancreatic islets were isolated from male young rats. Replicative was induced by serial passaging primary por...

Islet-2 is a LIM/homeodomain-type transcription factor of the Islet-1 family expressed in embryonic zebrafish. Two Islet-2 molecules bind to the LIM domain binding protein (Ldb) dimers. Overexpression of the LIM domains of Islet-2 or the LIM-interacting domain of Ldb proteins prevented binding of Islet-2 to Ldb proteins in vitro and caused similar in vivo defects in positioning, peripheral axon...

To understand the dynamics of islet population, especially during conditions with growth of the total islet mass, it is important to have reliable estimators of parameters describing the quantitative appearance of the islet population. We describe a stereological estimator of the volume-weighted mean islet volume based on unbiased assumption-free stereological principles. The volume-weighted me...

OBJECTIVE Cross-sectional studies have suggested that islet autoimmunity may be more prevalent in type 2 diabetes (T2D) than previously appreciated and may contribute to the progressive decline in β-cell function. In this study, we longitudinally evaluated the effect of islet autoimmune development on the progressive β-cell dysfunction in T2D patients. RESEARCH DESIGN AND METHODS Twenty-three...

The isolation of islets by collagenase digestion can cause damage and impact the efficiency of islet engraftment and function. In this study, we assessed the basement membranes (BMs) of mouse pancreatic islets as a molecular biomarker for islet integrity, damage after isolation, and islet repair in vitro as well as in the absence or presence of an immune response after transplantation. Immunofl...