Lactate dehydrogenase isoenzymes were partially separated by use of a previously described column technique for creatine kinase [Clin. Chem. 20, 36 (1974)]. Extracts of lactate dehydrogenase-rich tissues were used to evaluate column resolution. Samples layered on mini-columns containing DEAE-Sephadex were eluted with Tris-buffered sodium chloride (100 and 200 mmol/liter). Lactate dehydrogenase ...

Indoleacetic acid oxidase in tobacco callus cultures (Nicotiana tabacum L., cv. White Gold) was composed of at least two groups of isoenzymes, which were distinctly different in electrophoretic mobilities and in responses to growth substances. Indoleacetic acid had dual effects; at low concentrations it promoted the development of two fast-migrating indoleacetic acid oxidase isoenzymes, but at ...

Ewen, Lilian M.: Separation of alkaline phosphatase isoenzymes and evaluation of the clinical usefulness of this determination. Am. J. Clin. Pathol. 61: 142-154, 1974. A simple technic for separation of alkaline phosphatase isoenzymes on agarose gel, using thymolphthalein monophosphate as the isoenzyme indicator substrate, is described. Results demonstrate that separation of isoenzymes of alkal...

Lactate dehydrogenase C, an isoenzyme composed of C polypeptide subunits and found only in mature testes and spermatozoa, differs kinetically, chemically and immunologically from the five common isoenzymes of lactate dehydrogenase, each of which is a tetramer of A and/or B subunits. In the rat lactate dehydrogenase C exists in two molecular forms, isoenzymes C4 and A1C3. In addition to these tw...

p-Hexosaminidase (EC 3.2.1.30) is markedly increased in human serum in liver disease, chronic alcoholism, and pregnancy. Knowledge of the clearance rate of plasma! serum #{237}3-hexosaminidase is n cessary to evaluate this increase. We studied the plasma clearance of p3-hexosaminidase isoenzymes (purified from human serum and placenta) after their infusion into rat circulation. A recently devel...

Abstract The paper presents studies of the enzymatic activity barley grains different varieties. In experiments with varieties malting barley, isoforms amylolytic enzymes was determined at pH = 5.5, 7.0, 8.0 using a phosphate buffer system (1/15 M buffer). in germinated grain after removal sprouts and roots. to determine amylases study action acidic (pH 5.5), neutral 7) alkaline 8) environments...

Two isoenzymes of malate dehydrogenase (MDH) were demonstrated in plasmodia of Physarum polycephalum by polyacrylamide-gel electrophoresis. The more "cathodal" form was uniquely associated with mitochondria (M-MDH) and the other form was found in the soluble cytoplasm (S-MDH). The isoenzymes were separated by acetone fractionation of soluble plasmodial homogenates acidified to pH 5.0. The M-MDH...

Soluble arylsulfatase (EC 3.1.6.1) is present in the body fluids of man in the form of two isoenzymes, arylsulfatase A and B, which reportedly are useful biochemical markers for certain types of malignancy. However, rapid assay of the individual isoenzymes is extremely difficult; procedures based on differential inhibition or activation of the isoenzymes in a mixture yield only semiquantitative...