The mTOR pathway is a critical integrator of nutrient and growth factor signaling. Once activated, mTOR promotes cell growth and proliferation. Several components of the mTOR pathway are frequently deregulated in tumors, leading to constitutive activation of the pathway and thus contribute to uncontrolled cell growth. We performed a high-throughput screen with an isogenic cell line system to id...

confocal laser scanning microscopy (clsm) is a revolutionary advance in the field of light microscopy which, in recent years, has been used on interaction studies between host and pathogen. this study carried out on the flag leaves of the susceptible spring wheat cultivar (triticum aestivum l.) known as thatcher and its near isogenic line with the yr-18 adult-plant resistance gene to stripe rus...

Semi-dwarf genes have been used to alleviate lodging and increase harvest index in barley. The present study was conducted determine the effect of widely sdw1.d allele on agronomic characters. transferred from cv. Triumph Tokak 157/37 using marker assisted backcross breeding, a near-isogenic line developed. Parents + near isogenic were evaluated three rainfed, fall-planted seven spring-planted ...

Abstract BACKGROUND The topologically associated domain (TAD) on 8q24 surrounding MYC is important for many types of cancer. region also in the formation IDH-mutant glioma; risk allele rs55705857 being with development these tumors. significantly increased tumors an mouse model. Cerebral organoids have been found to recapitulate early brain. We hypothesized that may alter phenotype cerebral org...

"Inducibility" of amylase in Drosophila melanogaster was defined and investigated in a natural population from Japan. Inducibility represents the effects of factors remote from the structural gene that control the amount of enzyme produced. Inducibility of an isogenic line is measured as the ratio of the enzyme's specific activity in two different inducing environments. There was considerable g...

The effect of genotype-temperature interactions on body weight has been studied in a natural population of Drosophila melanogaster using four isogenic strains derived from it, and their hybrid F(1) and F(2) progenies. Measurements were made at four constant temperatures-25 degrees , 21 degrees , 17 degrees and 13 degrees C-and at a temperature oscillating between 17 degrees and 25 degrees C.-Lo...

MYH7 is a major gene responsible for hypertrophic cardiomyopathy (HCM). From patient's skin fibroblasts, we derived an iPSC line (CDGEN1.16) harboring the heterozygous R403L mutation, hot-spot codon in HCM. We subsequently corrected mutated using CRISPR/Cas9 editing and obtained isogenic control (CDGEN1.16.40.5) preserving genomic background of patient. Both lines were pluripotent could be effi...

To investigate the mechanism of nucleopolyhedrovirus resistance of silkworm, we bred a near-isogenic silkworm line, designated BC9, from the parental resistant strain NB and the susceptible strain 306, that is resistant to infection by nucleopolyhedrovirus. Proteomic techniques were employed to search for candidate genes playing a role in the antivirus response, based on differential protein ex...