In vitro ovarian follicle cultures may provide fertility-preserving options to women facing premature infertility due to cancer therapies. An encapsulated three-dimensional (3-D) culture system utilizing biomaterials to maintain cell-cell communication and support follicle development to produce a mature oocyte has been developed for the mouse. We tested whether this encapsulated 3-D system wou...

s:2022:"Background: Cryopreservation is an important option for preserving the fertility. We examined the reactive oxygen species (ROS) production, total antioxidant capacity (TAC) and developmental competence, of vitrified isolated mice pre-antral follicles as an alternative method for restoring fertility. Materials and Methods: The 140-160 μm pre-antral follicles mechanically isolated from ov...

background: the ultrastructural analysis of cultured follicles could direct us to understand subcellular changes during in vitro culture. objective: this study was done to verify the ultrastructural characteristics of in vitro cultured mouse isolated preantral follicles in co-culture system in the presence and absence of leukemia inhibitory factor (lif) materials and methods: mechanically isola...

The aim of the present study was to investigate the effects of activin-A and follistatin on in vitro primordial and primary follicle development in goats. To study primordial follicle development (experiment 1), pieces of ovarian cortex were cultured in vitro for 5 days in minimal essential medium (MEM) supplemented with activin-A (0, 10 or 100 ng/ml), follistatin (0, 10 or 100 ng/ml) or combin...

Background: Ovarian cryopreservation is an efficient way to preserve fertility of cancer patients before chemotherapy. This study was designed to evaluate the effect of apha lipoic acid (ALA) on reactive oxygen species (ROS) production and total antioxidant capacity (TAC) of cultured isolated pre-antral follicles derived from vitrified and non-vitrified ovarian tissue. Materials and Methods: Im...

BACKGROUND Autotransplantation of cryopreserved ovarian cortex can be associated with a risk of cancer cell reseeding. This issue could be eliminated by grafting isolated preantral follicles. Collagenase NB6 is an enzyme produced under good manufacturing practices (GMP) in compliance with requirements for tissue engineering and transplantation in humans and thus can be used to isolate preantral...

the aim of this study was to investigate the in vitro developmental competence of isolated pre-antral follicles derived from vitrified ovaries in the presence of coenzyme q 10 (coq 10 ) . mice pre-antral follicles derived from fresh and vitrified-warmed ovarian tissues were in vitro cultured individually in α-mem medium supplemented with or without coq 10 , followed by adding human chorionic go...

To completely avoid ice crystal formation and thus get a higher survival rate, vitrification methods have been commonly used for cryopreservation of oocytes and embryos. However, currently used vitrification methods for oocytes and embryos are not suitable for the cryopreservation of preantral follicles (PFs). In the present study, stainless steel mesh was fabricated into mini mesh cups to vitr...

Rat vibrissa dermal papillae were transplanted between the epidermis and dermis of isolated embryonic mouse skin and then grafted onto nude mice. The papillae induced the formation of hair follicles which were larger than those of the host skin but smaller than vibrissa follicles. The potential of isolated dermal papillae to induce follicles with characteristics of those from which the papillae...

Techniques for the isolation of ovarian follicles and maturation of oocytes in vitro have enormous reproductive potential. Preservation of normal tissue function is vital. This study emphasizes the ultrastructure and viability of mechanically isolated bovine small (diameter 40-100 microm) preantral and large (140-450 microm) preantral/early antral follicles. Viability studies were performed for...