• Modern techniques for extracellular vesicle isolation and separation are reviewed. Emphasis is given to modern size-, charged-, affinity-based approaches. A trend toward the combination of multiple EV isolation. Studies subpopulations require combinations techniques. Automated on-line technique allows quick reliable subpopulation studies. Extracellular vesicles (EVs) heterogenous membrane-bou...

A central goal of evolutionary biology is to identify the genes and evolutionary forces that cause speciation, the emergence of reproductive isolation between populations. Despite the identification of several genes that cause hybrid sterility or inviability-many of which have evolved rapidly under positive Darwinian selection-little is known about the ecological or genomic forces that drive th...

Routine techniques for the isolation of human peripheral blood mononuclear cells (PBMCs) include density centrifugation with Ficoll-Paque and isolation by cell preparation tubes (CPTs) and SepMate tubes with Lymphoprep. In a series of experiments, these three PBMC isolation techniques were compared for cell recovery and viability, PBMC population composition, and cell functionality, aiming to p...