Circulating immune complexes (CIC) were isolated from patients with ankylosing spondylitis (AS) and healthy blood donors by isopycnic ultracentrifugation in sucrose gradients. The CIC were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The major components of the CIC were identified as albumin, immunoglobulins, and complement factors. A 70 ...

The lipoproteins isolated from rat plasma by flotation in the density range 1.019-1.063 g/ml were further characterized. Using rate zonal ultracentrifugation, we isolated two lipoproteins in almost equal proportions from this density range. Similar isolations may be accomplished with density gradients in a swinging-bucket rotor. On isopycnic-densitygradient ultracentrifugation one component ban...

the techniques can easily make effective use of the most sophisticated of these methods." This admirable goal is partially achieved in this multiple-author collection. The chapters on velocity sedimentation (by D. Ridge), zonal rotors (by J.M. Graham), and isopycnic centrifugation (by G.D. Birnie and D. Rickwood) take the novice up to the point where he can apply the methods discussed in an int...

Separation of differentially isotope-labeled bacterial RNA by isopycnic density gradient centrifugation is a critical step in RNA-based stable isotope probing analyses, which help to link the structure and function of complex microbial communities. Using isotope-labeled Escherichia coli RNA, we showed that an 8 mL near-vertical rotor performed better than a 2 mL fixed-angle rotor, thereby corro...

The main goal of this study was to apply magnetic bead surface functionalization in the form immunomagnetic separation (IMS) combined with real-time polymerase chain reaction (qPCR) (IMS-qPCR) detect Human mastadenovirus species C (HAdV-C) and F (HAdV-F) water samples. technique efficiency compared a nonfunctionalized method (ultracentrifugation) followed by laboratory detection. Tests were car...

Abstract Background Small extracellular vesicles (sEVs) are nanosized involved in cell-to-cell communication. sEVs have been widely studied for clinical applications such as early detection of diseases and therapeutics. Various methods isolation using, but different may result qualities impact downstream analysis applications. Here, we compared current performed a comparative from supernatant c...

A micro-enzymatic method was developed to measure total cholesterol (CHOL) and triglyceride (TG) in lipoproteins and their subfractions separated by density gradient ultracentrifugation. This method had a detection limit and sensitivity below 2 mg/dl and accuracy (bias to reference sera) and imprecision (coefficient of variation) of less than 3% between 2 and 30 mg/dl for both CHOL and TG. In a...