Solitary ascidians have a long legacy as model organisms for investigating developmental mechanisms because of the simplicity of their embryogenesis and their basal phylogenetic position within the chordates (Berrill, 1929; Cameron et al., 2000; Conklin, 1905; Jeffery and Swalla, 1997; Kowalevsky, 1866; Satoh, 1994). The chordate traits that unite ascidians with the cephalochordates and vertebr...