s of papers 25 and β-glucanase, except a low level of α-amylase activity (85,000 U/gm vs. 19,000,000 U/gm pure α-amylase). Results of chick growth at 21 d showed positive (P < 0.05) main effects of CP level and enzyme supplementation on BW and FCR. Birds receiving diets with higher CP content had higher BW (800 gm at 23% vs. 722 gm at 17% CP, P < 0.05). Birds fed diets supplemented with keratin...

Feather keratin is highly resistant to degradation, but some keratinase producing microorganisms can easily degrade these insoluble keratins. These keratinase producing species have an important application in removal of poultry waste and recycled into valuable byproduct. Bacillus sp was screened from soil samples of slaughterhouse and poultry farm area using azokeratin medium. Highest keratina...

Feather keratin is highly resistant to degradation, but some keratinase producing microorganisms can easily degrade these insoluble keratins. These keratinase producing species have an important application in removal of poultry waste and recycled into valuable byproduct. A feather-degrading bacterium with high keratinase activity was isolated and identified as Bacillus megaterium (A1). The sel...

Feathers are byproduct waste of poultry processing plant and produced in large amount. A small percentage of feather waste is steamed, chemically treated, ground, to form feather meal a dietary protein supplement for animals. Alternatively, keratin can be biodegraded by some Keratinolytic bacteria and in this study Keratinase producing bacteria and their Keratinolytic enzyme production was inve...

A keratinolytic protease, secreted as the major component by a feline clinical isolate of Microsporum canis cultivated in a minimal medium containing cat keratin, was purified by affinity chromatography on bacitracin agarose and gel filtration. The apparent molecular mass of the enzyme was 31.5 kDa and the pI was 11.8. The enzyme was not glycosylated and its first 15 N-terminal amino acids show...

The Chryseobacterium sp. kr6 strain has been described as a highly keratinolytic bacterium showing effective feather-degrading and de-hairing activities. A keratinase Q1 enzyme was purified from Chryseobacterium sp. kr6 culture by Phenyl Sepharose and Superose 12HR chromatography. This enzyme showed a specific activity of 967U/mg for keratin azure. Electrophoresis under denaturing conditions sh...

Feeding trials were designed to evaluate the nutritive value of feather meal treated by K6 and K82 keratinase. There were five treatments in feather meal preparation: CFM (non-enzymatically treated feather meal), K6FM (K6 keratinase treated feather meal), K82FM (K82 keratinase treated feather meal), K6:K82FM [K6 and K82 keratinase (5:1) treated feather meal] and CMFM (commercial enzyme treated ...

The present study deals with isolation, identification and characterization of feather degrading bacterium. The keratinolytic bacteria were isolated from feather dumped soil. The colonies showed higher keratinase production was identified as Bacillius sp, as per Bergey’s manual method. Keratinase producing Bacillus sp, showed higher enzymes production. The crude filtrate showed specific activit...

The main keratinase (kerA) gene from the Bacillus licheniformis S90 was optimized by two codon optimization strategies and expressed in Pichia pastoris in order to improve the enzyme production compared to the preparations with the native kerA gene. The results showed that the corresponding mutations (synonymous codons) according to the codon bias in Pichia pastoris were successfully introduced...

Indonesia possesses tremendous marine resources. Therefore, their products are appropriate for exploration. In the prior study, bacteria generating keratinase enzyme have isolated from local fish market trash. The may hydrolyze keratin on skin. Surrounding parameters, such as temperature, pH, and incubation duration, factors affecting activity of enzyme. This study aims to isolate characterize ...