this study was conducted to determine the location of dna segment with homology to the rat conserved genomic dna in human chromosomes. the labeled rat genomic dna was hybridized with normal human (male) metaphases. the study of 74 metaphases after fluorescence in situ hybridization showed 371 twin-spot signals on human chromosomes. statistical analysis indicated that the specific accumulation o...

degradation of dry matter (dm) and nitrogen (n) in fresh or frozen-thawed alfalfa and ryegrass was studied by using in situ technique. the forages were labeled with 15n during growth in a glasshouse, harvested at similar growth phase, and fresh (f) or frozen-thawed (ft) samples were incubated in the rumen of 3 sheep. there was no difference (p>0.05) between forage type (s) for the immediately s...

We report a supersandwich fluorescence in situ hybridization (SFISH) strategy for highly sensitive and selective in situ visualization of mRNA expression patterns at the single-cell level. This strategy uses two fluorophore-labeled signal probes to generate a supersandwich product, which in turn generates numerous signal probes located at the target mRNA position, resulting in the in situ fluor...

We present a fluorescence-based, multiplex in situ hybridization method that permits the simultaneous detection of five differently labeled antisense RNA probes and up to seven differ-ent transcripts in a single Drosophila embryo. We also show that it should be possible to increase the number of detected transcripts substantially with nascent transcript multiplex fluorescent in situ hybridizati...

The gene encoding alpha fetoprotein (locus symbol Afp) was assigned to rat chromosome 14 at band p21-p22 using fluorescence in situ hybridization method. The present result suggests that there is a conserved syntenic group between human 4q11-q13, mouse 5F-G, and rat 14p21-p22.