Rates of formation and dissociation of an abortive ternary complex between lactate dehydrogenase isozymes from various mammalian tissues, NADf, and pyruvate were studied utilizing stopped-flow spectrofluorometry. Formation of the complex was accompanied by a decrease in protein fluorescence at 340 rnp and a loss of lactate dehydrogenase activity. Rates of formation of an abortive lactate dehydr...

The activity of lactate dehydrogenase of the supernatant fraction of brain, heart, skeletal muscle, and liver of l-day and 4-, 12-, 30-, 74-, and 96-week old rats was determined to study the changes in this enzyme with increasing age. Units of enzyme per g, wet weight, of all the tissues increased during the growth phase, reaching a maximum at 30 weeks, and then decreased. NADH,:NADHn ratios, K...

introduction: in some intoxicated patients, cerebrospinal fluid (csf) is examined due to the prolonged loss of consciousness, focal neurologic findings, and fever of unknown origin. we aimed to evaluate the probable relationship between the different toxicity causes and the csf indices in poisoned patients and determine if they could predict the patients’ outcome. methods: all patients who had ...

background tracking various biomarkers in serum, gingival crevicular fluid (gcf), and saliva has been introduced as a diagnostic tool for periodontal disease detection. objectives the aim of this study was to compare salivary lactate dehydrogenase (ldh) levels in subjects with periodontal disease and levels in subjects without periodontal disease. conclusions salivary ldh levels can be used as ...

in the present study, changes of values of serum 17ß-estradiol (e2), asparate amino teransferase (ast), alanine amino transferase (alt) and lactate dehydrogenase (ldh) following intraperitoneal (i.p) injection with the aromatase inhibitor (ai) letrozole were investigated in rainbow trout (oncorhynchus mykiss). forty- eight apparently healthy rainbow trout (mean weight of 845 g) divided in four ...

Procedures were developed for the optimal solubilization of D-lactate dehydrogenase, D-mandelate dehydrogenase, L-lactate dehydrogenase and L-mandelate dehydrogenase from wall + membrane fractions of Acinetobacter calcoaceticus. D-Lactate dehydrogenase and D-mandelate dehydrogenase were co-eluted on gel filtration, as were L-lactate dehydrogenase and L-mandelate dehydrogenase. All four enzymes ...

1. Papadopoulos, N. M., and Hufnagel, C. A., Evaluation of lactate dehydrogenase isoenzyme patterns in serum of patients undergoing cardiac surgery. J. Thorac. Cardiovasc. Surg. 76, 173-177 (1978). 2. McKenzie, D., Clarke, P. I., and Henderson, A. R., How accurate are lactate dehydrogenase isoenzyme estimations by the thin-layer agarose fluorescent technique? Clin. Chem. 22, 1995-1998 (1976). 3...

1. Papadopoulos, N. M., and Hufnagel, C. A., Evaluation of lactate dehydrogenase isoenzyme patterns in serum of patients undergoing cardiac surgery. J. Thorac. Cardiovasc. Surg. 76, 173-177 (1978). 2. McKenzie, D., Clarke, P. I., and Henderson, A. R., How accurate are lactate dehydrogenase isoenzyme estimations by the thin-layer agarose fluorescent technique? Clin. Chem. 22, 1995-1998 (1976). 3...

It has been assumed that the in vivo reduction of 3-mercaptopyruvate, an intermediate of cysteine metabolism, to 3-mercaptolactate is catalyzed by lactate dehydrogenase (EC 1.1.1.27) though no definitive evidence has been presented. In order to examine this assumption, reduction of 3-mercaptopyruvate and its inhibition were studied using rat liver homogenate, lactate dehydrogenase purified from...