OBJECTIVE To explore the use of β-lactoglobulin polymerized using microbial transglutaminase and heating to identify whether protein polymerization could reduce in vivo allergenicity and maintain in vitro and ex vivo immunoreactivity for use in tolerance-induction protocols. METHODS Based on previous protocols applied in mice and children, we performed in vivo challenges (using a skin prick t...

The fractal structure of the aggregates in food protein gels was analyzed. Three kinds of food protein gels were prepared: (1) β-lactoglobulin (β-LG) gel; (2) 11S soybean globulin gel; and (3) caseinate gel. From the concentration dependence of the gel elasticity, the fractal dimensions Df of the aggregates in the gels were evaluated, according to the theory of Shih et al. These gels showed the...

The transglutaminase-catalysed incorporation of primary amines (putrescine and monodansylcadaverine) into bovine beta-lactoglobulin has been studied. In the presence of 1 mM-dithiothreitol between 1 and 2 mol of amine can be incorporated per mol of beta-lactoglobulin subunit. There is very little incorporation of amines in the absence of reducing agent. By isolating and sequencing the modified ...

The concentrations of beta-lactoglobulin was measured in the sera of 47 preterm an term neonates during the first few days of life under standardised conditions after feeding with a cows' milk'based formula. Preterm neonates, particularly those of less than 33 weeks' gestation, had higher serum concentrations of beta-lactoglobulin than term neonates given an equivalent mild feed. Prior feeding ...

as 'goat lactoglobulin' gave a single peak on electrophoresis at pH 8.0 in barbiturate buffer, but three boundaries separated at pH 7-0 and at least two at pH 4-2-6-0 after 2 hr. at 4-5 v/cm., I= 01. The two main components had their isoelectric points at pH 5-2 and 5-9 respectively in sodium acetate buffer, I= 0-1. 'Goat lactoglobulin' thus behaved in much the same way as crystalline bovine ,B...

Depending on solution conditions, beta-lactoglobulin can exist in one of its six pH-dependent structural states. We have characterized the acid and basic-induced conformational transitions between these structural states over the pH range of pH 1 to pH 13. To this end, we have employed high-precision ultrasonic and densimetric measurements coupled with fluorescence and CD spectroscopic data. Ou...

Mammary explants from pregnant ewes were cultured in the presence of insulin, cortisol and prolactin, either alone, or in combination. After 2 d of culture, total RNA was extracted from explants and the content of beta-lactoglobulin mRNA was estimated using a specific labelled cDNA probe. The mRNA for beta-lactoglobulin was only deinduced slowly in the absence of hormone during the culture. Pro...

The study was conducted to find the association of -Lactoglobulin locus with the milk performance traits in 157 lactating Sahiwal cows maintained at Cattle yard, NDRI, Karnal. Two different variants of -Lactoglobulin were detected from DNA by PCR-RFLP. It was found that the frequency of B allele was predominantly high (0.84± 0.03) than that of A allele (0.16± 0.03) in the population studied. ...

High expression and secretion of recombinant ovine beta-lactoglobulin has been achieved in the yeast Kluyveromyces lactis. The yield of beta-lactoglobulin is 40-50 mg per litre of culture supernatant and accounts for approx. 72% of the total secreted protein. Constitutive expression is under the control of the Saccharomyces cerevisiae phosphoglycerate kinase promoter from an intronless version ...

I3C-NMR studies of modified 6-lactoglobulins, cyanylated at the single thiol group, have provided evidence to support the location of the thiol at Cys-121 [ I J and useful information about the nuclear relaxation behaviour of the thiocyanate carbons of cyanylated thiol groups in proteins [2]. The O-lactoglobulin monomer contains two tryptophan residues. Trp-19 is buried deep inside the hydropho...