BACKGROUND Leptospirosis is a re-emerging infectious disease that is under-recognized due to low-sensitivity and cumbersome serological tests. MAT is the gold standard test and it is the only serogroup specific test used till date. Rapid reliable alternative serogroup specific tests are needed for surveillance studies to identify locally circulating serogroups in the study area. METHODS/PRINC...

A rapid Listeria detection method was developed utilizing A511 bacteriophage amplification combined with surface-enhanced Raman spectroscopy (SERS) and lateral flow immunochromatography (LFI). Anti-A511 antibodies were covalently linked to SERS nanoparticles and printed onto nitrocellulose membranes. Antibody-conjugated SERS nanoparticles were used as quantifiable reporters. In the presence of ...

A simplified lateral-flow assay for the detection of antibodies to HIV using magnetic-bead conjugates and multibranched peptides from both HIV-1 and HIV-2 was developed. Magnetic immunochromatography testing (MICT) uses a standard lateral-flow platform that incorporates magnetic-bead conjugates for quantitative measurement of the magnetic field distortion associated with the bound magnetic conj...

Introdução: Os testes rápidos imunocromatográficos, de fácil execução e resultado rápido, configuram importante ferramenta no diagnóstico da SARS-CoV-2, porém sua taxa eficácia é variável a depender do fabricante, sendo necessário identificar os fatores que podem influenciar o desempenho destes quando comparados ao padrão-ouro, RT-PCR. Objetivo: Explanar as principais evidências sobre dos rápid...

Introduction: Worldwide, one of the important causes of childhood hospitalization is diarrhea, with rotavirus being commonly implicated. ELISA is usually used to diagnose the rotavirus infection in microbiology laboratory. It is time consuming and requires sophisticated equipments. We have compared a rapid Immunochromatography test with ELISA in our study. Aim and objective: To detect the prese...

AbstractAIMS: Aims: The Coronavirus Disease 2019 (COVID-19) caused not only global pandemic, but it also pointed at unprepared health care systems and countermeasures were introduced under the pressure of urgent circumstances. This review is focused on discussion critical evaluation instrumental tools for COVID-19 diagnosis that developed in last months. METHODS: Survey actual literature scient...

A lateral-flow immunoassay (LFT) was developed to detect bovine rotavirus in fecal samples. Using samples (n = 74) from diarrheic calves, a comparison of the LFT with a commercial latex agglutination test (LAT) and transmission electron microscopy (EM) was conducted. When EM was used as the reference method, initial studies of 29 samples indicated 70 and 80% sensitivities of the LFT and LAT, re...