Legionnaires' disease is a severe form of pneumonia, with worldwide relevance, caused by Legionella spp. Approximately 90% of all cases of legionellosis are caused by Legionella pneumophila, but other species can also be responsible for this infection. These bacteria are transmitted by inhalation of aerosols or aspiration of contaminated water. In Spain, environmental studies have demonstrated ...

The dynamics of Legionella spp. and of dominant bacteria were investigated in water from a cooling tower plant over a 9-month period which included several weeks when Legionella pneumophila proliferated. The structural diversity of both the bacteria and the Legionella spp. was monitored by a fingerprint technique, single-strand conformation polymorphism, and Legionella spp. and L. pneumophila w...

Legionella pneumophila, hafif seyirli pnömoniden, ağır pnömoni ve çoklu organ yetmezliğine kadar değişebilen klinik seyir gösteren, toplum hastane kaynaklı pnömonilere neden olabilen, sporadik veya salgınlar şeklinde ortaya çıkabilen bir bakteriyel etkenidir. akla getirilmezse tanıya yönelik testler istenmezse gözden kaçabilir. Burada, Covid -19 pandemisi döneminde acil servise yüksek ateş, öks...

We developed a rapid thermocycling, real-time detection (also known as real-time PCR) method for the detection of Legionella species directly from clinical specimens. This method uses the LightCycler (Roche Molecular Biochemicals, Indianapolis, Ind.) and requires approximately 1 to 2 h to perform. Both a Legionella genus PCR assay and Legionella pneumophila species-specific PCR assay were desig...

Legionella pneumophila is the causative pathogen of Legionnaires' disease, which is characterized by severe pneumonia. In regard to the pathophysiology of Legionella infection, the role of inflammatory phagocytes such as macrophages has been well documented, but the involvement of dendritic cells (DCs) has not been clarified. In this study, we have investigated the immune responses that DCs gen...

BACKGROUND Recurrent legionella outbreaks at one and the same location are common. We have identified a single Legionella pneumophila genotype associated with recurrent Legionella outbreaks over 6 years. METHODS Field emergency surveys following Legionella outbreaks were performed on a vessel in 2008, 2009 and 2013. Water samples from both the distribution and technical parts of the potable w...

background community acquired pneumonia (cap) is a common health concern and the main mortality factor worldwide. nowadays, legionella pneumophila is one of the most common microorganisms responsible for cap. we designed this study to investigate the antigen test as a useful, simple and rapid test for early diagnosis of legionella pneumophila. materials and methods we enrolled 118 patients (32 ...

Several strains of Legionella pneumophila and other species of Legionella with proteolytic activities were compared by assays, including Southern hybridizations and Western immunoblots, to determine their proteolytic, hemolytic, and cytotoxic activities. Only proteases from strains of L. pneumophila were both hemolytic and cytotoxic, and proteolytic activities extracted from other species of Le...

OBJECTIVES To determine the presence of legionellae and species composition of the genus Legionella in Sri Lankan hotel cooling towers, and to determine the previous exposure of hotel workers to Legionella pneumophila. DESIGN Collection of water samples from 16 cooling towers of air conditioning plants from 7 representative hotels, and blood samples from hotel workers. SETTING Department of...

over a period of three months (summer), 108 samples of water collected from four hospitals in te-hran. they were taken from numerous potable and non-potable water, including main supply cis-terns, calorifiers, hot and cold tap water, shower, cooling tower and drain of air condition system. culturing of concentrated and non-concentrated water samples on buffered charcoal yeast extract (bcye) aga...