Mycelial growth rates are presented for 11 strains of Lentinula edodes and six strains of Lentinula boryana cultivated on solid media: derived from malt extract (MEA); malt yeast extract (YMEA); and, YMEA plus soluble lignin derivatives (YMEA+WSLD). The results were compared with data for mycelial growth rates, of the same strains cultivated on substrates derived from wheat straw treated at dif...

The shitake mushroom (lentinula edodes) industry in the Gobi Desert region of southern Xinjiang has experienced rapid development and reached a certain scale. To clarify laws governing different cooling methods greenhouses identify suitable for production region, this study focused on monitoring environmental changes using three methods: natural ventilation cooling, water-sprinkling roof fan pa...

The basidiomycete Lentinula edodes (Le.) cytochrome P450, Le.CYP1 was functionally expressed in Saccharomyces cerevisiae. The microsomal fraction containing Le.CYP1 was prepared from the recombinant yeast and the Le.CYP1 was analyzed. The 7-ethoxycoumarin and benzo(a)pyrene were found to be the substrates of Le.CYP1 enzyme. Le.CYP1 converted 7-ethoxycoumarin to 7-hydroxycoumarin.

The 474-bp long terminal repeat (LTR) of the basidiomycete Lentinula edodes retrotransposon was found to contain a different type of transcription termination-poly (A)-addition signal on each strand: TATGT- - -TATG- - -TCT, first reported in yeast genes, and an AATAA (or ACATAA)- - -GT(Py)-rich sequence, frequently found in mammalian genes.

Antibacterial activity of Lentinula edodes against Bacillus subtilis was evaluated in cell-free filtrates obtained after growth in 14 different culture media. The highest B. subtilis growth inhibition was promoted by filtrates of growth media supplemented with rice bran, vermiculite or molasses. L. edodes dry mycelial biomass in liquid culture with 0.5% added rice bran was 3.2 mg/ml, after grow...

The ethanolic extract of Lentinula edodes was partially analyzed and then characterized for its efficacy in treating atopic dermatitis. Polyphenols were determined to be the major antioxidant component in the extract (6.12 mg/g), followed by flavonoids (1.76 mg/g), β-carotene (28.75 μg/g), and lycopene (5.25 μg/g). An atopic dermatitis (AD) model was established and epidermal and dermal ear thi...

Lentinula edodes (L. edodes), which imparts various health benefits to humans, is considered a novel source of soluble dietary fiber (SDF). In this study, ultrasonic-assisted hot-water method was used extract SDF (U-SDF) from L. edodes, and physicochemical, antioxidant hypoglycemic properties the U-SDF were investigated. Physicochemical showed that water solubility, holding capacity, swelling o...

Olga M. Tsivileva, Alexei N. Pankratov, Valentina E. Nikitina and Lidiya V. Garibova Laboratory of Microbiology and Mycology, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Entuziastov Avenue, Saratov 410049, Russia Department of Chemistry, N. G. Chernyshevskii Saratov State University, 83 Astrakhanskaya Street, Saratov 410012, Russia Depa...

Lignocellulosic residues were tested as artificial log to mushroom production of Lentinula edodes. Three isolates of L. edodes were grown in substrates based on corn bog, and sawdust or bark of eucalypt, enriched with rice bran 20%. The biological efficiency (BE) were higher when corn borg and sawdust was used and the isolate UFV 73 was that presented well shaped mushrooms and higher productivi...