نتایج جستجو برای: leukocyte rolling

تعداد نتایج: 51377  

2002
Martin Steinhoff Patricia Andrade-Gordon Nathalie Vergnolle Claudia K. Derian Michael R. D'Andrea Michael R. D’Andrea

Journal: :FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2001
E E Eriksson X Xie J Werr P Thoren L Lindbom

Leukocyte infiltration in atherosclerosis has been extensively investigated by using histological techniques on fixed tissues. In this study, intravital microscopic observations of leukocyte recruitment in the aorta of atherosclerotic mice were performed. Interactions between leukocytes and atherosclerotic endothelium were highly transient, thereby limiting the ability for rolling leukocytes to...

2015
Claudia Nussbaum Sarah Bannenberg Petra Keul Markus H Gräler Cassiano F Gonçalves-de-Albuquerque Hanna Korhonen Karin von Wnuck Lipinski Gerd Heusch Hugo C de Castro Faria Neto Ina Rohwedder Joachim R Göthert Vysakh Pushpa Prasad Günter Haufe Baerbel Lange-Sperandio Stefan Offermanns Markus Sperandio Bodo Levkau

Sphingosine-1-phosphate (S1P) participates in inflammation; however, its role in leukocyte rolling is still unclear. Here we use intravital microscopy in inflamed mouse cremaster muscle venules and human endothelial cells to show that S1P contributes to P-selectin-dependent leukocyte rolling through endothelial S1P receptor 3 (S1P3) and Gαq, PLCβ and Ca(2+). Intra-arterial S1P administration in...

2002
Martin Steinhoff Patricia Andrade-Gordon Nathalie Vergnolle Claudia K. Derian Michael R. D'Andrea Michael R. D’Andrea

Journal: :American journal of physiology. Heart and circulatory physiology 2005
Stephan C Schäfer Desiree N Sehrt Markus Kamler Heinz Jakob Hans-Anton Lehr

In contrast to acute preparations such as the exteriorized mesentery or the cremaster muscle, chronically instrumented chamber models allow one to study the microcirculation under "physiological" conditions, i.e., in the absence of trauma-induced leukocyte rolling along the venular endothelium. To underscore the importance of studying the naive microcirculation, we implanted titanium dorsal ski...

Journal: :Journal of immunology 2001
M Sperandio S B Forlow J Thatte L G Ellies J D Marth K Ley

L-selectin is a calcium-dependent lectin on leukocytes mediating leukocyte rolling in high endothelial venules and inflamed microvessels. Many selectin ligands require modification of glycoproteins by leukocyte core2 beta1,6-N-acetylglucosaminyltransferase (Core2GlcNAcT-I). To test the role of Core2GlcNAcT-I for L-selectin ligand biosynthesis, we investigated leukocyte rolling in venules of unt...

Journal: :Microcirculation 2009
Maria K Pospieszalska Alexander Zarbock John E Pickard Klaus Ley

OBJECTIVES P-selectin binding to P-selectin glycoprotein ligand-1 (PSGL)-1 mediates leukocyte rolling under conditions of inflammation and injury. The aims of this study were to develop an efficient, high temporal resolution model for direct simulation of leukocyte rolling and conduct a study of load-bearing bonds using the model. MATERIALS AND METHODS A stochastic pi-calculus-driven event-tr...

2002
Fernando Queiroz Cunha Bruno Fortes Maria Aparecida Oliveira Sérgio Henrique Claudia Farias Benjamim João Santana João Santana Silva Zuleica Bruno Fortes Sérgio Henrique Ferreira

Journal: :Blood 1993
K Ley G Linnemann M Meinen L M Stoolman P Gaehtgens

Leukocyte rolling in venules is inhibited by several sulfated polysaccharides, by antibodies to the leukocyte adhesion receptor L-selectin (LECAM-1), and by recombinant soluble L-selectin. The sulfated fucose polymer fucoidin and the polyphosphomannan PPME bind to L-selectin and inhibit L-selectin-mediated lymphocyte adhesion to lymph node high endothelial venules (LN-HEV). We investigated whet...

Journal: :The Journal of biological chemistry 2012
Caroline Spertini Bénédicte Baïsse Olivier Spertini

P-selectin glycoprotein ligand-1 (PSGL-1) mediates the capture (tethering) of free-flowing leukocytes and subsequent rolling on selectins. PSGL-1 interactions with endothelial selectins activate Src kinases and spleen tyrosine kinase (Syk), leading to α(L)β(2) integrin-dependent leukocyte slow rolling, which promotes leukocyte recruitment into tissues. In addition, but through a distinct pathwa...

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