نتایج جستجو برای: lexsy expression system
تعداد نتایج: 2986708 فیلتر نتایج به سال:
the human papillomavirus l1 major capsid protein (hpv l1), the basis of the current vaccines, self-assembles into virus-like particles (vlps). herein, we describe the expression and purification of recombinant hpv16 l1 in e. coli system. the l1 protein was generated in a fused form using an inducible expression system. the recombinant gst-l1 fusion protein migrated as a 82 kda protein in sds-pa...
this paper reviews recent strategies used for increasing specific yield and productivity in high cell density cultures. high cell density cultures offer an efficient means for the economical production of recombinant proteins. however, there are still some challenges associated with high cell density cultivation (hcdc) techniques. a variety of strategies in several aspects including host design...
conclusions in this experimental study, for the first time in iran the expression and purification of this recombinant protein was done successfully. this recombinant protein could be used as a vaccine candidate and diagnostic purpose in subsequent investigations. results the highest expression of the cytochrome p450 cyp141 protein was obtained by the addition of 1 mm of isopropyl β-d-1-thiogal...
background : amelogenins are the major components of enamel matrix proteins. enamel matrix derivatives (emd) can be used in periodontal diseases to regenerate periodontal tissues. the main aim of this study was to evaluate ex-pression of full-length functional recombinant human amelogenin (rham) in iranian lizard leishmania (i.l.l.) as an alternative eukaryotic expression system. methods : huma...
background and objectives: baculovirus can be used as a vector in gene delivery system. viral envelope of baculovirus would display expressed protein/peptide and it could render as a potential vaccine delivery system. in this regard, the gene coding for a subunit of shiga toxin (stxa) from escherichia coli ( e. coli ) strain was cloned in a baculovirus expression system. stxa subunit has the ab...
Abstract Background: The cysteine rich region II of Plasmodium vivax Duffy Binding Protein (PvDBP-II) is essential during merozoite invasion into the human erythrocyte and because of this biological importance, PvDBP-II is a vaccine candidate and a target for protective immunity against vivax malaria. In the present study we improved the yield of this protein by using E. coli (M15/BL21) expr...
background: today, despite the many advances in early detection of diseases, cancer patients have a poor prognosis and the survival rates in them are low. recently, microarray technologies have been used for gathering thousands data about the gene expression level of cancer cells. these types of data are the main indicators in survival prediction of cancer. this study highlights the improvement...
چکیده : نظام انتخاباتی قواعد و چار چوب های به رسمیت شناخته شده ای است که بیانگر چگونگی تبدیل آرای مردم به مناصب و پست های سیاسی است. این نظام انتخابات باید به گونه ای عمل کند که نتیجه آن فراهم شدن مکانیسم و ساز و کار مطلوب و مناسب برای برگزاری انتخابات سالم و رقابتی باشد. به نظر می رسد نظام انتخاباتی جمهوری اسلامی ایران دارای ضعف ها و نواقصی است که به سبب آن ها نمی توان انتخابات مطلوب را برگزا...
Human Interferon β (INF-β) is a member of cytokines family which different studies have shown its immunomodulatory and antiviral activities. In this study an expression vector was designed and constructed for expression of human INF-β-1b either in shake flasks and bench top bioreactor. The designed vector was constructed based upon pET-25b(+) with T7 promoter. Recombinant human beta interferon ...
abstract background and objective: agr is the most important regulatory system for the expression of staphylococcus aureus virulence factors in different conditions. agr acts as a quorum sensing system in this bacterium which is activated by increased cell concentration during the transition from logarithmic growth phase to stationary phase. its role is to upregulate the secretory virulen...
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