The paper-based immunoassay for point-of-care diagnostics is widely used due to its low cost and portability over traditional lab-based assays. Lateral-flow immunoassay (LFA) is the most well-established paper-based assay since it is rapid and easy to use. However, the disadvantage of LFA is its lack of sensitivity in some cases where a large sample volume is required, limiting its use as a dia...

Abstract Delamination of the interlayers multi-layer systems can cause a degradation in functionality, stability and life span that system. This is even more pertinent for at high temperature. Laser flash analysis (LFA) has long been used thermophysical properties measurements temperatures. Multi-layer reference artefacts, with without, debonded regions are required validating thermal character...

Signaling through the TCR as well as engagement of costimulatory molecules are required for efficient T cell activation and progression into differentiated effector cells. The beta2 integrin LFA-1 (CD11a/CD18) has been implicated in TCR costimulation as well as in cell-cell adhesion function, but its exact role is still ambiguous. The present study focuses on the requirement for LFA-1 in CD8+ T...

Abstract Background Angiotensin-converting enzyme (ACE) plays a major role in blood pressure regulation and cardiovascular homeostasis. The wide distribution multifunctional properties of ACE suggest it’s involvement various pathophysiological conditions. Results In this study, novel visual detection method for I/D polymorphisms was designed by integrating direct PCR without the need DNA extrac...

Several studies agree that microglial cells derive from monocytes that infiltrate the central nervous system during development, but the precise mechanism by which these cells enter into the nervous tissue is still unknown. In this way, the aim of the present study was to analyze the expression of two cell adhesion molecules involved in the recruitment of blood leukocytes into tissues, the lymp...

LFA-1 activation plays a major role in reducing the velocity of rolling leukocytes and causing firm adhesion to the endothelium. The signaling cascades that cause LFA-1 activation during this process have been described through mostly correlative experiments, but the precise occurrence and location of LFA-1 activation in response to specific signals has not been directly shown. Tim Springer’s g...

ICAMs are ligands for LFA-1, a major integrin of mononuclear cells involved in the immune and inflammatory processes. We previously showed that endothelial cell specific molecule-1 (ESM-1) is a proteoglycan secreted by endothelial cells under the control of inflammatory cytokines. Here, we demonstrate that ESM-1 binds directly to LFA-1 onto the cell surface of human blood lymphocytes, monocytes...

The involvement of the lymphocyte function-associated antigen-1 (LFA-1) membrane molecule in cytolytic T lymphocyte (CTL) interactions with lymphoid target cells was investigated using CTL clones derived from two patients with a heritable deficiency of LFA-1. LFA-1 surface expression on the CTL clones was 1% of the normal level of LFA-1, unchanged with prolonged culture, and identical on 14 dif...

LFA-1 and LFA-3 expression is absent or low on Burkitt's lymphoma cell lines and low on the EBV-transformed B cell line UD61. Incubation of cells of BL2 and of UD61 with various concentrations of IL-4 resulted in induction of LFA-1 and LFA-3 expression in a dose dependent fashion. This effect was already observed after 16 h of incubation whereas maximal expression was obtained after 72 h. Induc...