This article summarizes the basic principles of light microscopy, with examples of applications in biomedicine that illustrate the capabilities of the technique. Handbook of instrumental techniques from CCiTUB Advanced Light Microscopy Techniques 1 BT.4

Polarized light is the light in which all the rays vibrate in one plane. A polarizing microscope has two disk accessories. They are made up of polarizing plastic that allows light vibrating in one plane to pass. One of them is called a polarizer (placed below the condenser). Another similar disc is placed in the top part of the microscope and cuts off all the light vibrating in a perpendicular ...

To study the development and interactions of cells and tissues, multiple fluorescent markers need to be imaged efficiently in a single living organism. Instead of acquiring individual colours sequentially with filters, we created a platform based on line-scanning light sheet microscopy to record the entire spectrum for each pixel in a three-dimensional volume. We evaluated data sets with varyin...

Non-contact optical techniques for 3D surface data acquisition like short coherence (white light) interferometry, optical profilers or fringe projection are well established for applications with low surface complexity, where the average surface roughness amplitude is small and neglectable compared to the form amplitudes or where the aspect ratios of amplitudes and spacings are small. In genera...