Expressed sequence tags simple sequence repeats (EST-SSRs) are important sources for investigation of genetic diversity and molecular marker development. Similar to genomic SSRs, the EST-SSRs are useful markers for many applications in genetics and plant breeding such as genetic diversity analysis, molecular mapping and cross-transferability across related species and genera. In spite of low po...

We have cloned a SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) gene from Medicago truncatula (MtSERK1) and examined its expression in culture using real time PCR. In the presence of the auxin 1-naphthaleneacetic acid (NAA) alone, root differentiation occurs from the proliferating calli in both the cultured highly embryogenic seed line (2HA) and a low to nonembryogenic seed line (M. truncatula cv...

Medicago truncatula is attacked by a broad range of insect herbivores including sieve-element feeders, cell content feeders, and chewing insects. In M. truncatula, genetic resistance against insects has to date been identified only against aphids: single dominant resistance genes have been identified conferring resistance against the pea aphid, bluegreen aphid, and spotted alfalfa aphid. Aphids...

Phosphorus (P)-deficiency is a major abiotic stress that limits legume growth in many types of soils. The relationship between Medicago and Sinorhizobium, is known to be affected by different environmental conditions. Recent reports have shown that, in combination with S. meliloti 2011, Medicago truncatula had a lower symbiotic efficiency than Medicago sativa. However, little is known about how...

While some workers have studied cytochemical localizations during oogenesis in molluscs (e.g. Arvy, 1949, 1950; Bretschneider & Raven, 1951; Collier, 1960; Cowden, 1961; Pasteels & de Harven, 1963), others have examined the ultrastructure of the developing oocyte using the electron microscope (e.g. Rebhun, 1956a, b, 1961; Worley & Hershenov, 1960; Pasteels & de Harven, 1963). This work has been...

Biomass yield, salt tolerance and drought tolerance are important targets for alfalfa (Medicago sativa L.) improvement. Medicago truncatula has been developed into a model plant for alfalfa and other legumes. By screening a Tnt1 retrotransposon-tagged M. truncatula mutant population, we identified three mutants with enhanced branching. Branch development determines shoot architecture which affe...

The 14-3-3 gene family, which is conserved in eukaryotes, is involved in protein-protein interactions and mediates signal transduction. However, detailed investigations of the 14-3-3 gene family in Medicago truncatula are largely unknown. In this study, the identification and study of M. truncatula 14-3-3-family genes were performed based on the latest M. truncatula genome. In the M. truncatula...

Legume plants form nitrogen-fixing nodules on their roots in symbiosis with soil bacteria rhizobia. The number of symbiotic is controlled by a host plant via systemic mechanism known as autoregulation nodulation (AON). key players AON are the CLE peptides which produced root response to rhizobia inoculation and transported xylem shoot. In shoot, recognized CLV1-like receptor kinase, results sub...

As large numbers of metacercariae of Fasciola hepatica are necessary for research, experimental infections of Galba truncatula and Pseudosuccinea columella with this digenean were carried out to determine the better intermediate host for metacercarial production and, consequently, the most profitable snail for decreasing the cost price of these larvae. Pre-adult snails (4 mm in shell height) or...