transforming growth factor betas are multifunctional polypeptides in the cytokine superfamily. they have a growth inhibitory role on hemopoietic progenitor cells in semisolid colony assay as well as in long-term bone-marrow culture. tgf - β2 represses stromal cells, stem cell factor gene transcription, and decreases the stability of c-kit transcripts in hemopoietic cells. tgf-β also modulates g...

Chinese hamster ovary (CHO) cells are considered as the most commonly used host for industrial manufacturing of therapeutic proteins. The aim of this study was to evaluate the minimum inhibitory concentration (MIC) of hygromycin B in both CHO-DG44 and CHO-S cells since hygromycin B resistance cassette can be used for future selection of gene expression in CHO cells. The minimum inhibitory conce...

background: nigella sativa has been a nutritional flavoring factor and natural treatment for many ailments for so many years in medical science. earlier studies have been reported that thymoquinone (tq), an active compound of its seed, contains anticancer properties. previous studies have shown that tq induces apoptosis in breast cancer cells but it is unclear the role of p53 in the apoptotic p...

In this paper we are to present a practical application of Axiomatic Design (AD) methodology, as a roadmap to lean production, in redesigning a car body assembly line. Axiomatic Design theory provides a framework to simplify the whole problem. According to the AD principles, a hierarchical structure has been developed. The developed structure originated in lean manufacturing principles and exis...

abstract background and aims: to identify and isolation of viruses a susceptible cell is need which can be primary or line. the use of primary cells is laborious, time consuming and expensive, while the use of cell lines is much easier and faster. irkhbk cell line was originated from primary cells of bovine kidney by continuous passage. the aim of this study was to introduce the irkhbk cell lin...

most of the erythrocyte related diseases are detectable by hematology images analysis. at the first step of this analysis, segmentation and detection of blood cells are inevitable. in this study, a novel method using a line operator and watershed algorithm is rendered for erythrocyte detection and segmentation in blood smear images, as well as reducing over-segmentation in watershed algorithm t...

cancer cell-stromal cell interaction plays a crucial role in the growth and invasion of tumor cells of various organs. it has been recently suggested that adipocytes and preadipocytes, two specific types of stromal cells, affect the biological behavior of variety of breast cancer cell lines. in spite of few investigations, there is a controversy in the literatures about the effects of adipocyte...

Transforming growth factor betas are multifunctional polypeptides in the cytokine superfamily. They have a growth inhibitory role on hemopoietic progenitor cells in semisolid colony assay as well as in long-term bone-marrow culture. TGF - β2 represses stromal cells, stem cell factor gene transcription, and decreases the stability of c-kit transcripts in hemopoietic cells. TGF-β also modulat...

in this syudy a continuous bovine kidney cell line derived from a primary bovine kidney cells was established for the first time in iran. the cells were originating from two-day-old normal male calf of holstein breed. the cell cultures were continuously passaged following complete proliferation of primary cells. the specific properties or characteristics of the cell were defined using cytogene...