conclusions the findings of this study indicated that these isolates were plant pathogenic fungi, which entered seawater from the environment, and were likely to have a suitable lipase activity on plant oils. results four fungal species were isolated from water samples of the caspian sea (north of iran) between february and june 2011. the nucleotide sequences reported for three of these isolate...

A lipolytic pathway involving natriuretic peptides has recently been discovered in human fat cells. Its functional characteristics and the interactions of the atrial natriuretic peptide (ANP)-induced effects with adrenergic and insulin pathways were studied. Characterization of the action of ANP antagonists, i.e., A71915, anantin, S-28-Y (Ser-28-Tyr, a synthesized peptide), and HS-142-1 (a micr...

Metagenomic cloning is a powerful tool for the discovery of novel genes and biocatalysts from environmental microorganisms. Based on activity screening of a marine sediment microbial metagenomic library, a total of 19 fosmid clones showing lipolytic activity were identified. After subcloning, 15 different lipolytic genes were obtained; their encoded proteins showed 32-68% amino acid identity wi...

To investigate the role of the monounsaturated n-9 fatty acids (MUFA) in the lipolytic activity of adipocytes, a study was carried out in which an increase in MUFA was produced in the tissues by two different methods; by the dietary enrichment of oleic acid or by producing an essential fatty acid deficiency syndrome. For this, forty-five male Sprague-Dawley rats were fed with a normal-energy di...

1. Lipolytic and proteolytic activities and pH values were determined in secretions collected from innervated abomasal pouches and in abomasal contents from preruminant calves given liquid diets. 2. No lipolytic activity was detected in pouch secretions collected during 1 h after feeding, though lipolytic activity was present in abomasal contents; pepsin (EC 3.4.23.1) and renin (EC 3.4.23.4) we...

Hormone-sensitive lipase (HSL) is presumed to be essential for lipolysis, which is defined as the mobilization of free fatty acids from adipocytes. In the present study, we investigated the effects of various lipolytic hormones on the lipolysis in adipocytes derived from mouse embryonic fibroblasts (MEF adipocytes) prepared from HSL-deficient mice (HSL-/-). HSL-/- MEF differentiated into mature...

Proteins of fat cells, isolated from rat epididymal fat pads, were labeled with 14C-histidine or 14C-leucine. The fate of the labeled protein was examined after subsequent treatment of the cells with hormones. Release of labeled proteins was stimulated by lipolytic hormones (adrenocorticotropic hormone, glucagon, and epinephrine) and by theophylline. Proteolysis, as measured by the appearance i...

The lipolytic activity of bovine thyroid-stimulating hormone and its subunits have been compared to the lipolytic activity of ovine-luteinizing hormone and its subunits. When tested on white adipocytes isolated from epididymal fat pads of BALB/c mice, thyroid-stimulating hormone was a potent lipolytic agent, active at a concentration of 7 X lo-lo M. In contrast, the isolated subunits of thyroid...

Physical exercise accelerates the mobilization of free fatty acids from white adipocytes to provide fuel for energy. This happens in several tissues and helps to regulate a whole-body state of metabolism. Under these conditions, the hydrolysis of triacylglycerol (TG) that is found in white adipocytes is known to be augmented via the activation of these lipolytic events, which is referred to as ...

The role of Ca2+ in adrenocorticotropic hormone (ACTH)stimulated lipolysis in rat adipocytes was investigated using perifused fat cells. With this system, it is possible to monitor minute-to-minute changes in glycerol release from adipocytes during both the activation and inactivation phases of hormone-induced lipolysis. The lipolytic response to ACTH was dose-dependent and 0.1 unit per ml of A...