conclusions cd5l might be a useful biomarker for early diagnosis of hcc in hcv cirrhotic patients. lrg and hp α2 isoforms could be potential markers for distinguishing viral hcc. our results also further support the presence of varying molecules involved in hepatocarcinogenesis in hbv when compared with hcv infection. patients and methods two-dimensional polyacrylamide gel electrophoresis combi...

An isotope dilution liquid chromatography/tandem mass spectrometry is proposed as a reference method to determine the level of tyrosine in human serum. The advantages of this method include simple sample preparation without derivatization, the selective detection of compounds of interest in complex matrices, and the use of an isotopically labeled analogue as an internal standard. Tyrosine and i...

Chromatography coupling to mass spectrometry becomes a vital instrumentation tool for the separation and determination of small molecules qualitatively and quantitatively. In this study, the author is focused on the mitragynine and 7hydroxymitragynine. Mitragynine and its major metabolite, 7-hydroxymitragynine (also known as “kratom”), are drugs which extracted from a tree planted in Thailand, ...

In a growing number of laboratories the technique of liquid chromatography-tandem mass spectrometry is used for the quantification of cyclosporin A in whole blood, employing cyclosporin D as the internal standard. Cyclosporin A is extensively metabolized in vivo; in liquid chromatography-tandem mass spectrometry respective metabolites can give rise to both parent and product ions that are isoba...

Across evolution, dolichols and polyprenols serve as sugar carriers in biosynthetic processes that include protein glycosylation and lipopolysaccharide biogenesis. Liquid chromatography coupled with electrospray ionization mass spectrometry offers a powerful tool for studying dolichols and polyprenols in their alcohol or glycan-modified forms in members of all three domains of life. In the foll...